基于慈竹转录组MYB基因的克隆及胁迫诱导表达

Cloning of MYB gene based on Bambusa emeiensis transcriptome and their induced expression under stresses

从慈竹笋转录组数据库中克隆出2个MYB基因(Be MYB1、Be MYB2)进行生物信息学分析,探讨其响应脱落酸(ABA)、Na Cl、聚乙二醇6000(PEG 6000)胁迫的机制。结果表明,Be MYB1与Be MYB2基因分别编码632个和338个氨基酸;Be MYB1蛋白属于MYB相关蛋白,与小麦Ta MYB48蛋白聚为一枝,具有2个序列模体(motif);Be MYB2蛋白属于R2R3-MYB蛋白,与毛竹Pe MYB2蛋白、水稻Os MYB18蛋白聚为一枝,具有3个motif。Be MYB1和Be MYB2基因均响应了ABA、Na Cl和PEG 6000,但Be MYB2基因对胁迫的响应能力更强,Be MYB1和Be MYB2基因在响应非生物胁迫时发挥重要作用。

According to the transcriptome data of Bambusa emeiensis Chia et H.L.Fung‘Viridiflava ’ shoots, two MYB genes designated as BeMYB1 and BeMYB2 were cloned and their bioinformatics were analyzed .The mechanism of their response to abscisic acid （ ABA ） , sodium chloride （ NaCl ） and polyethylene glycol 6000 （ PEG 6000 ） stresses was explored .The bioinformatics analysis results showed that BeMYB1 and BeMYB2 genes encoded 632 and 338 amino acids respectively .BeMYB1 and wheat TaMYB48 proteins were clustered into the same branch , which were MYB-related proteins with two motifs .While BeMYB2 protein belonged to R2R3-MYB protein with three motifs , and it was aggregated into one branch with bamboo PeMYB 2 and rice OsMYB18 proteins.The stress induced expression analysis results displayed that BeMYB1 and BeMYB2 genes responded to ABA , NaCl and PEG 6000, but the response ability of BeMYB2 was higher than BeMYB1.Hence, it is indicated that BeMYB1 and BeMYB2 genes played an important role in the responses to abiotic stresses .