沙棘果对高脂膳食大鼠肝脏保护作用研究

Hepatoprotective Effects of Seabuckthorn in High-fat Diet Rats

探讨沙棘对高脂膳食大鼠的肝脏保护作用。以雄性Wistar大鼠60只为研究对象,随机分为对照组、高脂模型组和沙棘低、中和高剂量组。除对照组外,其余4组给予高脂饲料。对照组和高脂模型组灌胃蒸馏水;低、中和高剂量沙棘组分别灌胃不同剂量沙棘汁。喂养28 d后,腹主动脉取血,分离血清,测超氧化物歧化酶(SOD)活性、丙二醛(MDA)、丙氨酸氨基转移酶(ALT)和天冬氨酸氧基转移酶(AST)的含量,观察肝细胞超微结构变化。沙棘各剂量组与高脂模型组比较SOD活性均升高,MDA含量均降低,有显著性差异(P<0.05);高脂模型组与沙棘各剂量组比较,ALT和AST活性均降低,AST活性有统计学意义(P<0.05)。肝脏超微结构表明,沙棘能改善对高脂饮食所致的大鼠肝细胞超微结构的损伤。沙棘对高脂膳食大鼠肝细胞具有保护作用。

The purpose of this study was to examine the hepatoprotective effect of seabuckthorn in high-fat diet rats.Sixty male Wistar rats were randomly divided into five groups：the control group, the high-fat model group, the low-dose seabuckthorn group, the middle-dose seabuckthorn group and the high-dose seabuckthorn group. Rats were treated with high-fat diet besides the control group. Then rats in both control group and high fat model group was given distilled water and those in low, medium and high treated group were received different doses of seabuckthorn juice. After 28 days treatment, the blood on abdominal aorta was collected and the levels of serum superoxide dismutase （SOD）, malondialdelyde （MDA）, alanine aminotransferase （ALT）, aspartic acid oxo transferase （AST） were measured. And also the liver cell ultrastructure changes were observed. The each seabuckthorn dose group compared with high-fat model group, the SOD activity were increased, the MDA content were lower, and there was significant difference between two groups（P〈0.05）. The high-fat model group compared with each seabuckthorn dose group ALT and AST activity were reduced , the activity of AST was significantly difference between two groups（P〈0.05）. Liver ultrastructure showed that seabuckthorn can improve fat diet-induced liver cell damage in rats. In conclusion, seabuckthorn can play protective effects in high fat diet rat hepatocytes.