响应面法优化“酶法-超声波”连续提取核桃青皮多酚

Optimization of Enzymatic-ultrasonic Continuous Extraction of Polyphenols from Walnut Green Peels by Response Surface Methodology

为了优化核桃青皮中多酚类物质的"酶法-超声波"连续提取工艺,在单因素试验基础上,通过Design Expert8.05软件进行响应面设计与优化,得到了预测数学模型。结果表明,酶法提取最佳工艺条件为:采用纤维素酶,酶解温度55.00℃、酶解p H 5.00、酶解时间75.00 min、酶添加量15.00 U/m L、液料比30∶1 m L/g。酶解后继续采用超声波辅助二次提取,以50%"乙醇-水"为提取液,超声时间45 min、超声温度50℃、超声功率100 W,此条件下多酚提取率为61.36%。

In order to optimize the enzymatic-ultrasonic continuous extraction processes of polyphenols from walnut green peels,the optimum extraction technology and mathematical regression model were established by response surface methodology on the basis of single-factor test. The results showed that the optimal extraction conditions were extraction temperature of 55℃, extraction time of 75.00 min, extraction pH of 5.00, enzyme dosage of 15.00 U/mL, ratio of liquid to solid of 30∶1 mL/g. And the ultrasonic-assisted extraction was operated after enzymatic extraction for 45 min at the power of 100 W by 50%ethanol solution as extractant. Under these conditions, the maximum extraction yield of polyphenols from walnut green peels was 61.36%.