金叶败毒颗粒防治妊娠中期巨细胞病毒宫内感染的实验研究

Experimental Study of Prevention and Therapeutic Effect of Jinyebaidu Granules on Cytomegalovirus Infection During Mid-pregnancy

目的观察金叶败毒颗粒防治妊娠中期豚鼠巨细胞病毒宫内感染的效果。方法选择无感染史、性成熟期雌雄豚鼠同笼受精,随机选择妊娠中期母豚鼠,分为3组,每组15只,正常对照组无特殊处理,模型对照组腹腔接种病毒,金叶败毒组接种病毒同时灌胃给予金叶败毒颗粒(3.09 m L·kg-1),7 d后观察各组母豚鼠病毒血症发生率,20 d后观察各组胎盘感染率、胎仔感染率及死胎率。结果正常对照组和模型对照组死胎率分别为8.33%,34.55%(P<0.05)。模型对照组和金叶败毒组亲代感染率分别为86.67%,33.33%;胎盘感染率分别为91.67%,61.22%;胎仔感染率分别为90.91%,48.28%;死胎率分别为34.55%,15.52%(均P<0.05)。结论金叶败毒颗粒可减少妊娠中期病毒接种引起的母胎感染及胎仔死亡,减少胎盘感染。

Objective To study the effect of Jingyebaidu granules on treating cytomegalovirus （ CMV） infection during mid-pregnancy. Methods The sexually mature guinea pigs with no CMV infection history served as the subjects. Put the male and female ones in the same cages. Then the female ones were randomly divided into three groups during mid-pregnancy. Model control group：15 guinea pigs which were inoculated 1 mL suspension of GPCMV intraperitoneally. Jingyebaidu Medicine group：15 guinea pigs which were treated with Jingyebaidu（3. 09 mL·kg^-1 ） through stomach perfusion after inoculation for 14 days. Normal control group：15 normal mid-pregnant guinea pigs. Viremia rates were examined 7 days after infection. All animals were sacrificed 20 days after infection. The placenta infection rate, pup infection rate, still-born rate were examined. Results Compared with the normal controls, the still-born rate was increased in model control group（8. 33% vs 34. 55%, P〈0. 05）. In comparison to the model control group, the GPCMV maternal infection rate（86. 67% vs 33. 33%）, placenta infection rate （91. 67% vs 61. 22%）, pup infection rate（90. 91% vs 48. 28%）, still-born rate（34. 55% vs 15. 52%） were significantly decreased in the Jinyebaidu group （all P〈0. 05）. Conclusion Jinyebaidu granules could reduce maternal infection,pup loss, and placenta infection caused by CMV inoculation during mid-pregnancy.