苦橘咳喘合剂的止咳化痰作用与急性毒性

Eliminating Phlegm and Stopping Cough by Kujyukekuan Mixtures and Its Acute Toxicity

目的观察苦橘咳喘合剂的止咳化痰效果,评价其急性毒性。方法采用小鼠氨水引咳实验、小鼠酚红实验、二甲苯小鼠耳廓肿胀实验和最大耐受量实验。结果镇咳实验中苦橘咳喘合剂小、中、大剂量组R值分别为112.26%,143.30%,162.07%,分别呈现为无效、有效和显效;苦橘咳喘合剂大、中剂量组能显著增加小鼠气管酚红排泌量,与正常对照组比较差异有统计学意义(P<0.05或P<0.01),且呈良好量效关系;苦橘咳喘合剂小、中、大剂量组均能显著改善二甲苯所致小鼠耳廓肿胀度(P<0.01),且呈良好量效关系;其最大耐受量为326.76 g·kg-1。结论苦橘咳喘合剂中、大剂量具有一定的镇咳祛痰作用和良好的抗炎效果,且安全性较好。

Objective To study the relieving cough and reducing phlegm effects and acute toxicity of the Kujyukekuan mixtures. Methods The method used in our study is a cough model induced by the ammonia water , the phenol red test in mice, the mouse pinna swelling model induced by xylene and the maximum tolerant dose experiment. Results In antitussive experiments, R value of Kujyukekuan mixtures at low, medium and high dose of was 112. 26%, 143. 29%, 162. 07%, respectively regarding as ineffective, effective and marked effective;Kujyukekuan mixtures at medium and high doses increased the excretion of phenolsulfonphthalein in the trachea of mice （compared with the normal control group, P〈0. 05 or P〈0. 01）,the results showed good concentration-response relationship;Kujyukekuan mixtures at low, medium and high dose significantly reduced mouse auricle swelling caused by xylene （compared with normal control group, P〈0. 01）, the results presented a good concentration-response relationship;the MTD of Kujyukekuan mixture was 326. 76 g·kg^-1. Conclusion Kujyukekuan mixtures at medium and high doses exert significant eliminating cough and phlegm effects and strong anti-inflammatory property, and has a good security.