贵州产金耳环不同采收期多糖的含量变化及止血作用

Change of Polysaccharide from Asarum insigne in Guizhou During Different Harvest Period and the Hemostatic Effect of Polysaccharide Study

目的观察贵州产金耳环不同采收期多糖含量的变化及止血作用。方法利用水提醇沉法提取金耳环多糖,经除杂、纯化后,采用紫外分光光度法测定多糖含量;采用剪尾法及玻片法测定小鼠出、凝血时间。结果不同采收期金耳环中多糖含量具有明显差异,6月采收整体含量较高,为1.78%~1.82%。正常对照组和精制多糖大剂量组小鼠出血时间分别为（6.73±1.21）,（4.91±1.58）min（P〈0.01）;正常对照组和精制多糖中、大剂量组小鼠凝血时间分别为（7.27±2.09）,（3.96±1.78）,（3.27±1.61）min（P〈0.05或P〈0.01）。结论多糖是金耳环止血的活性物质;金耳环用于止血以6月采收为宜。

Objective To study the dynamic change of polysaccharide from Asarum insigne in Guizhou during different harvest period and its hemostatic effect. Methods Asarum insigne polysaccharide was extracted by water isolation and alcohol precipitation. We measured the polysaccharide content by UV spectrophotometry after impurity and purification and detected the bleeding and clotting time by tail cutting and slide methods in mice. Results There was significant variation in polysaccharide content of Asarum insigne at different harvest time, which was at a higher level in June（1. 78%-1. 82%）. The bleeding time in mice of normal control group was （6. 73±1. 21） min,and that in mice treated with refined polysaccharide at high dose was （4. 91±1. 58） min,the difference between two groups was statistically significant（P〈0. 01）. The clotting time in mice of normal control group was （7. 27±2. 09） min,and that in the refined polysaccharide at middle and high dose groups was （3. 96±1. 78） min and （3. 27±1. 61） min,respectively. The latter two groups were obviously different from the normal control group（P〈0. 05 or P〈0. 01）. Conclusion The polysaccharide is an active hemostatic substance in Asarum insigne and the optimum harvest time of it is in June for the clinical use.