艾灸各因素对APP/PS-1双转基因AD小鼠脑中DNA、蛋白质、脂质氧化应激的影响

Effects of different factors of moxibustion on DNA, protein and lipid oxidative stress in brain issue of APP/PS-1 transgenic AD mice

目的:通过对比艾灸各因素对APP/PS-1双转基因阿茨海默病(AD)小鼠大脑皮质中DNA、脂质和蛋白质氧化损伤产物的影响,探讨艾灸的抗衰老机制。方法:将5月龄C57BL/6小鼠作为空白对照组(6只),同龄APP/PS-1小鼠随机分为模型组、艾灸组、无烟灸组、艾烟组(6只/组)。艾灸与无烟灸组灸治小鼠关元穴。艾烟组小鼠束缚暴露于5-15mg/m3烟气环境,空白对照组与模型组小鼠束缚暴露于正常环境,20min/d,6d/周,共8周。于第9周取材,ELISA法检测大脑皮质中8-羟基脱氧鸟苷(8-Ohd G)、4-羟基壬烯醛(4-HNE)、3-硝化酪氨酸(3-NT)含量。结果:皮质中8-Ohd G、4-HNE、3-NT含量,模型组高于空白对照组(P<0.05);艾灸组、艾烟组、无烟灸组显著低于模型组(P<0.01)。结论:艾灸各因素可通过影响APP/PS-1小鼠脑内8-Ohd G、4-HNE、3-NT含量,改善脑内氧化应激状态,起到延缓脑组织衰老作用。

Objective： By comparing the effects of different factors of moxibustion on DNA, lipid and protein oxidized products in the cerebral cortex of APP/PS-1 AD mice, to investigate the potential anti-aging mechanism of moxibustion. Methods： Five-month old C57BL/6 mice （n=6） were used for normal control group. Same age APP/PS-1 mice were randomly divided into four groups （n=6）： model group, moxibustion group, smokeless moxibustion group, and moxa smoke group. Moxibustion was performed with a moxa stick on Guanyuan （RN4） for 20min per day, 6 days per week for a total of 8 weeks. In the smokeless moxibustion, the procedure was same except that moxa stick was exchanged by smokeless one. Mice of the moxa smoke group were exposed to smoke, whose concentration was from 5 to 15mg/m^3, however, the normal control mice were just exposed to normal environment. The mice were sacrificed and the tissues were harvested for ELISA test in the 9th week. Results： The level of 8-OHdG, 4-HNE, and 3-NT in the model group was higher than that in the normal control group （P〈0.05）; moxibustion group, smokeless moxibustion group, and moxa smoke group was lower than model group （P〈0.01）. Conclusion： Moxibustion, smokeless moxibustion, and moxa smoke may change the oxidative stress of brain via inhibiting the production of 8-OHdG, 4-HNE, 3-NT in cortex neurons, and delaying the brain aging.