芎麻汤药效部位干预硝酸甘油型偏头痛大鼠行为学症状、外周血及脑组织中5-HT的研究

Study on active extracts of Xiongma Decoction on behavioral symptoms and 5-HT in plasma and brain tissue on rat model of migraine with Nitroglycerin

目的:研究芎麻汤药效部位对硝酸甘油所致偏头痛大鼠行为学症状、血浆及脑组织中三叉神经颈髓复合体(TCC)上5-羟色胺(5-HT)的影响,初步探讨其药效部位的作用机制。方法:32只雄性SD大鼠随机分为空白组(A组),模型组(B组),芎麻汤药效部位组(C组),琥珀酸舒马普坦阳性对照组(D组),每组8只;采用皮下注射硝酸甘油(10mg/kg)复制偏头痛模型,观察大鼠挠头、爬笼、咬尾、往返运动,取颈外静脉血采用ELISA检测血浆中5-HT含量,心脏灌注后取大鼠TCC采用免疫组化检测5-HT的阳性表达。结果:芎麻汤药效部位能有效减轻偏头痛大鼠行为学症状,有效降低偏头痛大鼠血浆中5-HT含量,有效降低偏头痛大鼠TCC内5-HT免疫阳性染色光密度值,与模型组比较,差异具有统计学意义(P<0.05,P<0.01)。结论:芎麻汤药效部位能干预硝酸甘油诱导的偏头痛,其机制可能与调节体内5-HT水平有关。

Objective： To study the effects of active extracts of Xiongma Decoction on behavioral symptoms and levels of 5-HT in plasma and trigeminocervical complex （TCC） on animal model of migraine with Nitroglycerin. Methods： Thrity-two SD rats were randomly divided into 4 groups： blank group （group A）, model group （group B）, Xiongma Decoction group （group C）, and sumatriptan group （group D）. The migraine model was established by giving hypodermic injection of 10mg/kg Nitroglycerin, the scratching, climbing, biting, back and forth movement were observed, the content of 5-HT on plasma and the expression of 5-HT on TCC were detected by ELISA and stry method. Results： Compared with the model group, Xiongma Decoction could effectively reduce the behavioural symptoms of migraine rat, the content of 5-HT on plasma and positive immunostaining IOD of 5-HT on TCC in Xiongma Decoction group were decreased significantly （P〈0.05, P〈0.01）. Conculusion： Xiongma Decoction is effective on the Migraine rat induced by nitroglycerin, the mechanism may be related to adjusting the level of 5-HT.