姜黄素对大鼠脑缺血-再灌注损伤的影响及机制

Effect of Curcumin on Cerebral Ischemia-reperfusion Injury in Rats

目的:观察姜黄素对大鼠脑缺血-再灌注损伤的影响及其机制。方法:制作SD大鼠全脑缺血-再灌注损伤模型。实验设假手术组、模型组及姜黄素低、中、高剂量组,检测并比较各组大鼠脑组织损伤程度、白细胞浸润、神经元肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)及血脑屏障通透性随脑缺血-再灌注损伤时间表达变化的情况。结果:对应时间点,模型组及姜黄素组大鼠在脑损伤评分、白细胞数量、MMP-9表达、TNF-α表达及伊文思蓝含量均高于假手术组(P<0.05);姜黄素各剂量组脑损伤评分、白细胞数量、MMP-9表达、TNF-α表达及伊文思蓝含量显著低于相应模型组(P<0.05),以中剂量作用最佳。结论:姜黄素可减轻脑缺血病理损害,其机制可能与抑制神经元缺血-再灌注后白细胞的浸润、MMP-9、TNF-α表达及改善脑组织血脑屏障通透性有关。

Objective： To investigate the effect of curcumin on cerebral ischemia-reperfusion injury in rats and its mechanism. Methods ：250 male SD rats were randomly divided into five groups：sham group（ Sham group） , ischemia-reperfusion group （ L/R group） ,curcumin groups with dosage of 30 mg/kg（ Cur30 group） , 100 mg/kg（ CuriO0 group） and 3O0 mg/kg（ Cur300 group）. The brain tissue damage degree,leukocyte cells infiltration, levels of TNF-cz and MMP-9 expressions, and blood-brain barrier permeabili- ty were detected. Results ： At the same time point, the score of brain tissue injury, number of leukocyte, expression of MMP-9 and TNF- a and Evans blue dye of I/R group and Curs group were higher than those of Sham group （ P 〈 0. 05 ）. The score of brain tissue damage degree, number of leukocyte, expression of MMP-9 and TNF-a, and Evans blue dye of Cur groups were lower than those of I/R group（ P 〈 0.05 ）. The CuriO0 group had the best effect. Conclusion ： Curcumin can decrease cerebral isebemia reperfusion pathological damage significantly and suppressed the expression of MMP-9 and TNF-a, and Evans blue dye, brain tissue damage,leukocyte infiltration, which may be involved in protective mechanisms of curcumin.