大叶杨组培快繁体系的建立

Establishment of Tissue Culture System of Populus lasiocarpafrom Its Axillary Bud Induction

以大叶杨幼嫩的带腋芽茎段为试材,建立以腋芽诱导途径为基础的植株组培快繁体系。结果表明:大叶杨腋芽诱导的最适宜培养基为MS+NAA0.1 mg/L+6-BA0.1 mg/L,接种30 d,腋芽萌发率可达100%;最适宜增殖培养基为MS+NAA0.1 mg/L+6-BA2.0 mg/L,培养30 d,增殖系数可达4.0,幼苗生长健壮;最佳生根培养基为1/2MS+NAA0.1mg/L+IBA0.2 mg/L,培养30 d,平均生根达4条,平均根长为4.7 cm。

Using tissue culturing of young stem nodes with axillary buds,the rapid propagation system of Populus lasiocarpa was established.The results showed that the explants growing on pure MS medium had a germination rate of31.3%.The optimal medium for axillary bud induction was MS＋NAA0.1 mg/L＋6-BA0.1 mg/L,and on which the germination rate was 100% at the 30 th day after inoculation.The optimal medium for proliferation culture was MS＋NAA0.1 mg/L＋6-BA2.0mg/L,and on which the multiplication index was 4.0and the shoots were strong at the 30 th day after inoculation.The optimal medium for rooting was 1/2MS＋NAA0.1 mg/L＋IBA0.2 mg/L,and on which the rooting rate was 100% and the average root number was 4 and the average root length was 4.7 cm at the 30 th day after inoculation.