不同造模方法对大鼠多囊卵巢综合征伴胰岛素抵抗模型的影响

Influence of Different Model-Making Methods on the Rats Models Suffering from Polycystic Ovarian Syndrome and Insulin Resistance

目的:采用不同方法诱导SD清洁级雌性大鼠多囊卵巢综合征(PCOS)伴胰岛素抵抗(IR)动物模型,造模完成后检测大鼠血清相关激素、空腹血糖(FPG)及空腹胰岛素(FINS)并观察卵巢局部形态学改变。方法:分别采用来曲唑、脱氢表雄酮、左旋-十八甲基炔诺酮硅胶棒联合HCG诱导大鼠PCOS伴IR模型,ELISA法测定血清促黄体生成素(LH)、促卵泡生成素(FSH)、雌二醇(E2)、睾酮(T)、空腹胰岛素(FINS)水平,并用血糖仪测定FPG,HE染色后光镜下观察卵巢局部形态。结果:A组(对照组)和B组(来曲唑组)比较,B组较A组血清FSH、FINS及T浓度升高,差异有统计学意义(P<0.05);B组血清LH浓度较A组明显升高,差异有显著统计学意义(P<0.05)。D组(左旋-十八甲基炔诺酮硅胶棒联合HCG组)与C组(对照组)比较,D组血清LH、E2浓度较C组升高,差异有统计学意义(P<0.05);D组T浓度较C组明显升高,差异有统计学意义(P<0.05)。E组(脱氢表雄酮组)中途失败剔除。A、C 2组卵巢局部形态基本正常,可见发育成熟的卵泡及优势卵泡,B、D二组未见优势卵泡,均可见卵巢多囊样改变。结论:使用来曲唑或左旋-十八甲基炔诺酮硅胶棒联合HCG诱导大鼠PCOS伴IR模型,无论在影响血清激素及FPG、FINS,还是卵巢局部形态学改变方面与临床表现很接近,符合动物PCOS伴IR造模要求。

To test the relative hormones in serum, fasting plasma glucose （FPG）, fast insulin（FINS） and to observe local morphological changes of the ovary of rats after establishing SD rats models with polycystic ovarian syndrome（PCOS） and insulin resistance（IR） by using different inducing methods. Methods： Letrozole, dehydroepiandrosterone, silastic capsule of levonorgestrel subdermal together with HCG were used to induce rats models with PCOS and IR. ELISA method was adopted to test the levels of LH、FSH、E2、T and FINS in serum. Glucometer was used to test FPG. Local morphological structure of ovary was observed through light microscope after HE coloration. Results： Compared with group A（the control group）, the density of FSH、FINS and T increased in group B（induced by Letrozole） and the difference was of statistical significance（P〈0.05）. Compared with group A, the density of LH in serum of group B increased significantly and the difference was of great statistical significance（P〈0.05）. Compared with group C （the control group）, the densities of LH and E2 of group D（induced by silastic capsule of levonorgestrel subdermal together with HCG） increased and the difference was of statistical significance（P〈0.05）. Compared with group C, the density of T of group D increased significantly and the difference was of great statistical significance（P〈0.05）. Group E （induced by dehydroepiandrosterone） failed during the experiment and was deleted. In Group A and C, the local morphological structure of the ovaries were normal and mature follicles and dominant follicle could be seen while in group B and D, no dominant follicles could be seen and polycystic ovaries could be seen. Conclusion： In terms of the influence on serum hormones, FPG and FINS or the local morphological changes of the ovary, the rats models with PCOS and IR induced by Letrozole or silastic capsule of levonorgestrel subdermal together with HCG show similar symptoms as those in the clinic. So these methods meet the requirement of making animal models with PCOS and IR.