槲寄生药材中紫丁香苷薄层鉴别方法研究

Study on TLC Method for Identification of Syringin in HuJiSheng

目的:以紫丁香苷为对照品,建立槲寄生药材的薄层鉴别方法。方法:以紫丁香苷为对照品,根据《中国药典》(2010版)附录ⅥB(薄层色谱法)项下方法,对3个产地,共8批槲寄生药材进行薄层鉴别。考察不同提取方法、不同展开系统、不同点样量、不同薄层板、不同温度、不同湿度对槲寄生药材中紫丁香苷薄层鉴别的影响,从中选出槲寄生药材中紫丁香苷薄层鉴别的最佳条件。结果:以紫丁香苷为对照品,三氯甲烷-甲醇-水(15∶4∶0.2)为展开剂,10%硫酸乙醇溶液为显色剂,供试品和对照品在相同位置显紫色斑点。结论:该方法可行、专属性强、重复性好,能够作为槲寄生药材的鉴别方法。

To establish TLC method for identifyingHuJiSheng（HerbaVisci） by taking syringin as the reference substance. Methods： According to the methods recorded in ⅣB of appendix in Chinese Pharmacopoeia （2010）, syringin was carried out to be the reference substance to identify eight batches ofHuJiShengfrom three producing areas by TLC methods. In order to screen the best condition for identifying syringin inHuJiShengby TLC methods, the influence for TLC identification was investigated by analyzing the different extraction methods, developing systems, sampling amount, thinlayer plates, temperature and humidity. Results： The purple spots of test substance and reference substance appeared in the same position with syringin as reference substance, chloro-form-methanol-water（15：4：0.2） as developing solvent and 10% alcoholic solution of sulfuric acid as chromogenic reagent. Conclusion： The method, which is feasible, highly specific and reproducible, can be used to identify HuJiSheng.