臭蒿药材薄层色谱鉴别的方法学研究

The methodology research of Artemisia hedinii Ostenf. with TLC

目的建立藏药臭蒿的质量控制方法。方法本文初步建立了臭蒿药材的薄层色谱鉴别方法,以东莨菪内酯为指标成分,以乙酸乙酯-甲醇-浓氨试液(17:2:1)为展开剂,展开,取出,晾干后,置紫外光灯(365nm)下检视。结果供试品色谱中,在与对照品色谱相应的位置上,显相同的蓝色荧光主斑点。结论该方法操作简单,稳定性好。为臭蒿的质量标准建立提供一定的依据。

Objective To establish a method for quality control of Tibetan virgate wormwood.Method This paper first establishes the method of TLC identification of Artemisia hedinii Ostenf.Scopoletin is seen as the reference component. Using the liqiuid consisted of ethyl acetate-methanol-concentrated ammonia solution（17： 2： 1） as the developing solvent. After on, remove and dry.Then it can be examined under a uv lamp （365 nm）. Results It shows the same blue fluorescence spots in the corresponding position of the reference substance. Conclusion This method is simple and steady. It provides a certain basis for the establishment of the quality standards of the Artemisia hedinii Ostenf..