HPLC法同时测定美洲大蠊中尿嘧啶与肌苷的含量研究

Content Determination of Uracil and Inosine in Periplaneta americana by HPLC

目的建立美洲大蠊药材中尿嘧啶与肌苷含量测定的方法。方法采用HPLC法对尿嘧啶与肌苷的含量同时进行测定,色谱柱选用waters sunfire TM C18柱（4.6×250 mm;5μm）,以3%甲醇溶液（含0.07%醋酸）为流动相A,甲醇为流动相B,流速为0.6 ml·min^-1,检测波长为254 nm,柱温为25℃。结果尿嘧啶对照品在0.020 3-0.202 8μg范围内与吸光度呈良好线性关系（r=0.999 96）,加样回收率为99.90%（RSD=2.61%,n=6）;肌苷对照品在0.103 76^-1.037 6μg范围内与吸光度呈良好线性关系（r=0.99999）,加样回收率为99.94%（RSD=2.25%,n=6）。结论该方法操作简单,重复性好,精密度较高,可作为美洲大蠊中尿嘧啶与肌苷的含量测定方法。

Objective To establish a method for content determination of uracil and inosine in Periplaneta americana. Methods Content determination of uracil and inosine in Periplaneta americana by HPLC,the waters sunfire TM C18（4.6×250 mm; 5μm）was selected to be the chromatography column, with 3% aqueous methanol（containing 0.07% acetic acid） as the mobile phase A, methanol as mobile phase B, the flow rate is 0.6 ml·min^-1, the detection wavelength was 254 nm, the column temperature is 25 °C. Results The uracil in the range of 0.020 3 -0.202 8 μg（r = 0.999 96）was with a good linear relationship, and the total polysaccharide was 99.90%（RSD = 2.61%, n = 6）; The inosine in the range of 0.103 76 - 1.03 76 μg（r = 0.999 99）was with a good linear relationship, and the total polysaccharide was 99.94%（RSD = 2.25%, n = 6）. Conclusion The method is simple and can be used for the determination of uracil and inosine in Periplaneta americana.