环境浓度三丁基锡对根田鼠体重及摄食相关行为的影响

Effects of Tributyltin(TBT)at Environment Concentrations on Bodyweight and Feeding Related Behaviors of Root Voles

为了探究环境浓度三丁基锡(tributyltin,TBT)对根田鼠体重及摄食相关行为的影响,实验组使用1μg/m L的TBT按5μL/(g·BW)每3 d 1次进行灌胃;对照组使用等剂量生理盐水做相同处理,实验持续45 d。记录根田鼠体重,并计算其摄食量和饮水量(供给-剩余);摄食、饮水和运动的持续时间和频次用录像记录。结果显示:TBT暴露下,雄性根田鼠的摄食频次和时间、饮水频次和时间仅在有限的几个时间点上显著高于对照组(P<0.05);对雌性根田鼠摄食和饮水的频次和时间的影响不明显。TBT暴露下,雄性根田鼠仅在灌胃第3天时其运动时间显著高于对照组(P<0.05)、静止时间显著低于对照组(P<0.05),而随着染毒时间的延长,两组之间再无显著性差异。与对照组相比,TBT暴露对雌雄根田鼠摄食量、饮水量和体重的影响均不明显。因此,环境浓度的三丁基锡对成体根田鼠的体重没有明显影响,但对其摄食相关行为有部分影响。

This study was conducted to investigate the effect of TBT at environment concentrations on the bodyweight and feeding related behaviors of root voles. The root voles of experimental group were administrated with 1μg/mL of TBT according to the dose of 5μL/（g＆#183;BW） once every 3 days by gavage, and those of control group were administrated with the same volume physiological saline. During the experiment period of 45 d, the body weight of the root voles was recorded and the amount of their food intake and drinking water （supply-surplus） were measured. Furthermore, the duration and frequency of feeding, drinking and movement were recorded with videotape. The results showed that only in a limited number of observation time points, the duration and frequency of feeding and drinking of male root voles in TBT exposure group were significantly higher than those in control group （P〈0.05）. However, there were not significant differences of the duration and frequency of feeding and drinking between female root voles in TBT exposure group and those in control group. The movement time of male root voles in the 3rd d after administrated with TBT was significantly more than that of the male root voles in control group （P〈0.05）, whereas the rest time was significantly less （P〈0.05）. With extension of the exposure time, none of significant differences of the movement and rest time were observed between the two groups. The influence of TBT exposure on the amount of food intake and drinking water and body weight of female root voles was not significant. It was indicated that the body weight of adult root voles was not significantly influenced by TBT exposure at environment concentrations, whereas the feeding related behaviors were partly influenced.