IBRV对牛单核巨噬细胞吞噬鸡红细胞功能的影响

Effect of IBRV on Phagocytizing Chicken Erythrocytes Function of Bovine Monocytemacrophage

为研究牛传染性鼻气管炎病毒(IBRV)感染对牛单核巨噬细胞吞噬功能的影响,应用淋巴细胞分离液获得牛外周血单个核细胞,通过贴壁培养成为单核巨噬细胞。IBRV感染单核巨噬细胞24 h后加入鸡红细胞,测定被感染细胞对鸡红细胞的吞噬率。结果显示,单核巨噬细胞被IBRV感染后,对鸡红细胞的吞噬率显著降低(P<0.05)。

To investigate the effects of infectious bovine rhinotracheitis virus （IBRV） infection on phagocytizing chicken erythrocytes function of bovine monocyte-macrophage, lymphocyte separating medium was used to isolate the bovine peripheral blood mononuclear cells. The isolated cells were subsequently cultured to monocyte-macrophage by adherent culture method. The chicken erythrocytes were added to bovine monocyte-macrophage which was infected by IBRV for 24 h, and the phagocytizing chicken erythrocytes rate of the infected cells was determined. The results showed that the phagocytic chicken erythrocytes rate of IBRV infected bovine monocyte-macrophage was significantly reduced （P〈0.05）.