肝再生磷酸酶-3蛋白与肝细胞癌门静脉癌栓形成的关系

Correlation between phosphatase of regenerating liver-3 and portal vein tumor thrombus of hepatocellular carcinoma

目的探讨肝再生磷酸酶-3（PRL-3）蛋白与肝细胞癌（肝癌）门静脉癌栓形成的关系。方法回顾性分析2003年12月至2008年12月在佛山市第一人民医院行肝切除的248例肝癌患者临床资料。所有患者均签署知情同意书,符合医学伦理学规定。其中男193例,女55例;年龄18～75岁,中位年龄48岁;合并门静脉癌栓45例。采用免疫组化法观察PRL-3蛋白在肝癌及门静脉癌栓中的表达。分析PRL-3蛋白表达与肝癌患者临床病理学参数的关系及其在肝癌门静脉癌栓形成中的意义。PRL-3蛋白表达与肝癌临床病理学参数的关系分析采用χ^2检验。影响门静脉癌栓形成的危险因素分析采用Logistic回归分析。结果 PRL-3蛋白在肝癌及门静脉癌栓中的阳性表达率分别为70%（174/248）、98%（44/45）,其在门静脉癌栓中的阳性表达率明显高于肝癌（χ^2=15.253,P〈0.05）。PRL-3蛋白阳性表达与肝癌患者的血清AFP、肿瘤直径、肿瘤组织学分化程度、门静脉癌栓形成、肿瘤TNM分期、早期复发有关（χ^2=4.836,7.186,4.226,5.357,13.862,10.824;P〈0.05）。PRL-3蛋白阳性表达是肝癌门静脉癌栓形成的危险因素（OR=2.674,P〈0.05）。结论 PRL-3蛋白在肝癌门静脉癌栓中表达升高,其阳性表达与肝癌的侵袭、转移相关。

Objective To explore the correlation between phosphatase of regenerating liver-3（PRL-3） and portal vein tumor thrombus in hepatocellular carcinoma（HCC）. Methods Clinical data of 248 patients with HCC undergoing hepatectomy in the First People＇s Hospital of Foshan between December 2003 and December 2008 were retrospectively analyzed. The informed consents of all patients were obtained and the local ethical committee approval had been received. Among the 248 patients, 193 were males and 55 were females with the age ranging from 18 to 75 years old and the median of 48 years old. Forty-five cases were complicated with portal vein tumor thrombus. The expression of PRL-3 in HCC and portal vein tumor thrombus were observed by immunohistochemistry. The correlation between the expression of PRL-3 and the clinicopathological parameters of HCC patients and its significance in portal vein tumor thrombus formation were analyzed. The analysis on the correlation between the expression of PRL-3 and the clinicopathological parameters of HCC was conducted using χ2 test and the analysis on the risk factors associated with portal vein tumor thrombus formation was conducted using Logistic regression analysis. Results The positiveexpression rate of PRL-3 in HCC and portal vein tumor thrombus was 70%（174/249） and 98%（44/45）respectively, and the positive expression rate in portal vein tumor thrombus was significantly higher than that in HCC（χ^2=15.253, P〈0.05）. The positive expression of PRL-3 was associated with the serum AFP, tumor diameter, histological differentiation of tumor, portal vein tumor thrombus formation, tumor TNM staging and early recurrence（χ2=4.836, 7.186, 4.226, 5.357, 13.862, 10.824; P〈0.05）. The positive expression of PRL-3was the risk factor for portal vein tumor thrombus formation（OR=2.674, P〈0.05）. Conclusions PRL-3expression increases in HCC portal vein tumor thrombus and positive expression of PRL-3 may be closely associated with the invasion and metastasis of HCC.