PKM2基因在非小细胞肺癌中表达及其与放疗敏感性的关系

Expression of pyruvate kinase M2 in non-small cell lung cancer and its correlation with radiation sensitivity

目的:研究PKM2在非小细胞肺癌组织中的表达及其与放疗敏感性的关系。方法:选取2011年5月至2013年10月四川省人民医院非小细胞肺癌120例患者,术后进行2个月的放射治疗,按照疗效将患者分为4组。RT-PCR检测患者放疗前手术切除的肿瘤组织和癌旁组织中PKM2 m RNA表达水平。Western blot和免疫组织化学检测肿瘤组织和癌旁组织中PKM2蛋白表达。RT-PCR和Western blot方法检测不同疗效组PKM2表达水平。结果:非小细胞肺癌放疗2个月后,治疗有效率为49.2%。PKM2在非小细胞肺癌癌组织中表达显著高于相应的癌旁组织。PKM2表达水平与患者放疗疗效呈负相关。结论:PKM2在非小细胞肺癌中表达高于癌旁组织,PKM2低表达患者对放疗敏感性高。

Objective： To investigate the expression level ofpyruvate kinase M2 （PKM2） in tissues of non-small cell lung cancer （NSCLC） patients and the correlation between PKM2 expression level and radiation sensitivity. Methods： A total of 45 NSCLC pa- tients were chosen and treated with radiotherapy for two months after surgery. The patients were classified into four groups based on the curative effect. The mRNA expression levels of PKM2 in tumor and the homologous paraneoplastic tissues of NSCLC patients were detected prior to radiotherapy using real time-polymerase chain reaction （RT-PCR）, and the protein expressions of PKM2 in the tumor and paraneoplastic tissues of NSCLC patients were detected with Western blot analysis and immunohistochemical method. The mRNA and protein expression levels of PKM2 in the tumor tissues of different groups were detected with RT-PCR and Western blot assays. Results： The effective rate of radiotherapy for 2 months is 44.8% in NSCLC patients. The expression level of PKM2 is significantly higher in tumor tissues than in homologous paraneoplastic tissues of NSCLC patients and is negatively correlated with the curative effect of radiotherapy. Conclusion： The expression level of PKM2 is significantly higher in tumor tissues than in the paraneoplastic tissues of NSCLC patients. Patients with lower PKM2 expression level are more sensitive to radiotherapy.