L~﹡a~﹡b~﹡色度系统评估硫酸镍斑贴试验结果分析

Analysis of the evaluation of the Lab chromatic system on the result of nickel sulfate patch test

目的：了解硫酸镍斑贴试验不同结果对L＊a＊b＊色度系统变化的影响。方法：对38名健康志愿者进行人体皮肤封闭型硫酸镍斑贴试验。将2．5％硫酸镍斑试器用胶带贴敷在受试者上背部，同时设空白对照，封包24h后揭去斑试物。分别于试验前（D0）、揭去斑试物后0．5h（D1）、24h（D2）对皮肤反应结果进行临床评估及皮肤颜色L＊a＊b＊值测定。结果：38例受试者中8例在D1和（或）D2时临床评估为阳性。镍斑贴试验阴性组、空白对照组受试者D1、D2时皮肤色度L＊值、b＊值及a＊值较D0均无明显变化；斑贴试验阳性组D1、D2时皮肤色度a＊值分别为10．64±1．90和13．22±1．92，较D0时皮肤色度a＊值7．80±2．49明显升高（P=0．000）。结论：硫酸镍斑贴诱发的接触性皮炎模型中，L＊a＊b色度系统a＊值能反映皮肤发生炎症后的红斑变化，可作为评估接触性皮炎的客观评价参数。

Objective To understand the effects of the different results of nickel sulfate patch test on the L＊a ＊b ＊chromatic system. Methods The occlusive human nickel sulfate patch test was in 38 healthy volunteers.The 2.5%nickel sulfate patch test was tapped on the back of the volunteers for 24 hours.Measurements including clinical evaluation and the determination of L＊a ＊b ＊were made before patch occlusion and 0.5h（D1）,24h（D2） after patch removal, respectively. Results In all 38 subjects, 8 cases were judged positive at D1 and （or） D2.There was no significant difference between positive group an negative group in the values of L＊＊b ＊and a＊at D1 and D2. The a＊values of positive goup at D1, D2 were 10.64±1.90,13.22±1.92 respectively, which both were higher than the value 7.80±2.49 before patch test （P=0.000）. Conclusion In the contact dermatitis model inducing by nickel sulfate, the a＊val？ue of the L＊a ＊b ＊chromatic system can reflect erythematic changes after skin inflammation, showing a＊value maybe the objective evaluation parameter to evaluate contact dermatitis.