电针结合天麻多糖对局灶性脑缺血大鼠丘脑腹后外侧核巢蛋白和干细胞因子表达的影响

Effects of electroacupuncture combined with polysaccharide of gastrodia elate blume on expression of nestin and stem cell factor in thalamic ventroposterolateral nucleus in rats with focal cerebral ischemia

目的:探讨电针结合天麻多糖治疗局灶性脑缺血继发丘脑损害的神经再生机制。方法:将40只成年SD大鼠随机分为正常组、模型组、电针组、天麻多糖组和电针+天麻多糖组,每组8只。以线栓法建立右侧大脑中动脉栓塞脑缺血大鼠模型。造模成功后2周,正常组和模型组不予治疗;电针组给予电针"百会"和左侧"足三里"穴治疗,30min/次,每天1次,共14d;天麻多糖组给予天麻多糖(100mg/kg)灌胃治疗14d,每天1次;电针+天麻多糖组予以电针和天麻多糖联合治疗,每天1次,共治疗14d。采用免疫组织化学方法检测各组大鼠丘脑腹后外侧核(thalamic ventroposterolateral nucleus,VPL)神经巢蛋白(nestin)和干细胞因子(stem cell factor,SCF)的表达。结果:模型组大鼠缺血侧VPL出现nestin阳性细胞,SCF阳性细胞数量较正常组增加(P<0.05);电针组、天麻多糖组和电针+天麻多糖组缺血侧VPL中nestin和SCF阳性细胞数量均较模型组增加(均P<0.05),免疫阳性产物单位面积平均灰度值均降低(均P<0.05);电针+天麻多糖组nestin和SCF阳性细胞数量均较电针组、天麻多糖组增多(均P<0.05)。结论:电针结合天麻多糖可协同增加缺血同侧VPL中SCF的表达,共同促进神经干细胞增殖,可能是针药结合治疗脑缺血继发丘脑损害的神经再生机制之一。

Objective To explore the nerve regeneration mechanism of electroacupuncture（EA）combined with polysaccharide of gastrodia elate blume（PGB）for secondary thalamic damage of focal cerebral ischemia.MethodsForty Sprague-Dawley adult rats were randomly divided into a normal control group,a model group,an EA group,a PGB group and an EA ＋ PGB group,8rats in each group.The rat model of right middle cerebral artery occlusion was prepared by suture-occluded method.Two weeks after model establishment,rats in the normal control group and model group received no treatment;rats in the EA group were treated with EA at＂Baihui＂（GV 20）and left＂Zusanli＂（ST 36）,30 min per treatment,once a day for 14 successive days;rats in the PGB group were treated with intragastric administration of PGB（100mg/kg）,once a day for 14days;rats in the EA ＋ PGB group were treated with EA and PGB treatment,once a day for totally 14 days.The expressions of nestin and stem cell factor（SCF）in thalamic ventroposterolateral nucleus（VPL）were detected by immunohistochemical method.Results There were positive cells of nestin in ischemia VPL in the model group,and the number of SCF positive cells was increased compared with that in the normal control group（P〈0.05）.The number of positive cells of nestin and SCF in ischemia VPL in the EA group,PGB group and the EA ＋ PGB group was increased compared with that in the model group（all P〈0.05）,and the average gray value of immune positive product was all reduced（all P〈0.05）.The number of positive cells of nestin and SCF in the EA ＋ PGB group was higher than that in the EA group or the PGB group（all P〈0.05）.Conclusion EA combined with PGB can significantly increase the SCF expression in ischemia VPL and promote the proliferation of neural stem cells,which is likely to be one of the nerve regeneration mechanism of acupuncture and medication for secondary thalamic damage of focal cerebral ischemia.