Photosan脂质立方液晶纳米光敏剂的制备及光动力杀伤效应研究

Preparation of Photosensitizer- loaded Cubic Liquid Crystalline and Its Photodynamic Therapy Effects

目的:制备Photosan脂质立方液晶纳米光敏剂,通过体外实验探讨其介导的光动力疗法(photodynamic therapy,PDT)特异性杀伤肿瘤细胞效果。方法:以单油酸甘油酯(glyceryl monooleate,GMO)和泊洛沙姆407(poloxamer 407,P407)为液晶材料,以光敏剂Photosan为模型药物,制备Photosan立方液晶纳米粒,通过Malvern粒径仪和扫描电子显微镜等考察其理化性质;通过MTS法考察Photosan立方液晶纳米粒对正常肝细胞株和肝癌细胞株的暗毒性及光动力杀伤效果。结果:成功制备Photosan脂质立方液晶纳米粒,该纳米粒对人肝L-02细胞和人肝癌Hep G2细胞均没有暗毒性,其介导的PDT对人肝L-02细胞增殖有一定抑制作用[细胞抑制率为(32.9±1.19)%],而对肝癌Hep G2细胞增殖具有更显著的抑制作用[细胞抑制率为(77.9±2.06)%];Photosan立方液晶介导的光动力作用对人正常肝细胞的增殖抑制作用低于Photosan,但对肝癌细胞的增殖抑制作用高于Photosan。结论:Photosan脂质立方液晶纳米粒对人正常肝细胞安全性较好,对肝癌细胞的光动力杀伤效应明显优于Photosan,为光动力治疗癌症提供了创新性方法。

Objectives： To prepare photosensitizers-loaded Liquid Crystalline Nanoparticles, and study its cytotoxicity and photodynamic therapy （PDT） effects on tumor cells. Methods： Photosan Cubosomes were prepared with Glyceryl Monooleate （GMO）, Poloxamer 407 （P407） and Photosan. Then the cubosomes were observed on the dynamic light scattering and scanning microscope. The photodymanic therapy effects and cytotoxicity of Photosan Cubosomes to L02 cells and HepG2 cells were studied by MTS assay. Results： Photosan Cubosomes were prepared successfully. The results showed that the blank cubosomes, Photosan and Photosan Cubosomes did not display any cellular toxicity against L-02 cells and HepG2 cells without laser irradiating; however, Liquid Crystalline Photosan mediated PDT by 5J/cm2 laser produced obvious inhibitory effects on HepG2 cells [inhibited （77.9±2.06）%] while showed slight inhibitory effects on L-02 cells [inhibited （32.9±1.19）%]. Moreover, Liquid Crystalline PhotosanPDT showed less damage on L-02 cells but stronger inhibitory effects on HepG2 than Photosan. Conclusions： New photosensitizer loaded Photosan Cubic Liquid Crystalline had higher security and stronger PDT effects on cancer cells and showed better characteristics than Photosan. It provided a novel method for cancer treatment by PDT.