白皮杉醇和乙酰化白藜芦醇对小鼠辐射损伤的防护作用

Protective effect of piceatannol and acetylated resveratrol on radiation damage in mice

目的探讨白藜芦醇(resveratrol)衍生物白皮杉醇(piceatannol)和乙酰化白藜芦醇(acetylated resveratrol)对小鼠辐射损伤的防护作用。方法将昆明小鼠随机分为8组:正常对照组、照射对照组、白皮杉醇低、中、高剂量(50、100、200 mg/kg)组和乙酰化白藜芦醇低、中、高剂量(50、100、200 mg/kg)组,每组6只。小鼠经5.5 Gy 60Coγ射线一次性全身照射前2 h灌胃给药,对照组均灌服含2%DMSO的生理盐水溶液。照射后24 h,观察小鼠脾脏和胸腺系数、外周血淋巴细胞数量和百分率、血浆超氧化物歧化酶(superoxide dismutase,SOD)活力及骨髓嗜多染红细胞微核率的变化。结果与正常对照组相比,照射对照组小鼠脾脏和胸腺系数、外周血淋巴细胞数量和百分率及血浆SOD活力均显著降低(P<0.05或P<0.01),骨髓嗜多染红细胞微核率显著升高(P<0.01)。与照射对照组相比,白皮杉醇和乙酰化白藜芦醇各剂量组脾脏系数均显著增高(P<0.05或P<0.01),但胸腺系数差异无统计学意义(P>0.05);乙酰化白藜芦醇中、高剂量组外周血淋巴细胞数量和淋巴细胞百分率均显著升高(P<0.05或P<0.01);白皮杉醇中、高剂量组以及乙酰化白藜芦醇各剂量组血浆SOD活力均显著升高(P<0.05或P<0.01);白皮杉醇及乙酰化白藜芦醇各剂量组骨髓嗜多染红细胞微核率均显著降低(P<0.05或P<0.01)。结论白藜芦醇衍生物白皮杉醇和乙酰化白藜芦醇均对小鼠具有一定的辐射损伤防护作用,其作用机制与提高机体免疫能力、清除活性氧(reactive oxygen species,ROS)、保护造血组织免于辐射诱发的损伤有关。

Objective To investigate the protective effect of piceatannol and acetylated resveratrol on radiation damage in mice. Methods Kunming mice were randomly divided into eight groups, six for each. The mice in normal control group received no radiation, while those in the other seven groups received a single systemic radiation with 5. fi Cy Gy60Coγ. The mice in normal control and model control groups were administered with physiological saline containing 2% dimethyl sulfoxide （DMSO） by lavage 2 h before radiation, while those in three piceatannol groups with piceatannol at dosages of 50, 100 and 200 mg/kg, and those in three acetylated resveratrol groups with acetylated resveratrol at dosages of 50, 100 and 200 mg/kg, respectively. The coefficients of spleen and thymus, count and percentage of peripheral blood lympho- cytes, activity of plasma superoxide dismutase （SOD） and micronucleus （MN） rate of polychromatic erythrocytes in bone marrow were determined 24 h after irradiation. Results Compared with those in blank control group, the coefficients of spleen and thymus, count and percentage of peripheral blood lymphocytes and plasma SOD activity of mice in model control group decreased significantly （P 〈 0. 05 or P 〈 0. O1 ）, while the MN rate increased significantly （P 〈 0. 01 ）. However, compared with those in model control group, the coefficients of spleen of mice treated with resveratrol or acetylated resveratrol at various dosages increased significantly （P 〈 0. 05 or P 〈 0.01 ）, white those of thymus showed no significant difference （P 〉 0. 05 ）. The counts and percentages of peripheral blood lymphocytes of mice treated with acetylated resvera-trol at moderate and high dosages increased significantly （P 〈 0. 05 or P 〈 0. 01 ）. The plasma SOD activities of mice treated with piceatannol at moderate and high dosages and acetylated resveratrol at various dosages increased significantly （P 〈 0. 05 or P 〈 0. 01 ）. The MN rates of mice treated with resveratrol or acetylated resveratrol at various dosages de- creased significantly （P 〈 0. 05 or P 〈 0. 01 ）. Conclusion Both piceatannol and acetylated resveratrol showed a certain protective effect on radiation damage in mice, of which the mechanism was associated with the improvement of immunity, clearance of reactive oxygen species （ROS） and protection of hemopoietic tissues against radiation-induced damage.