miR-98对肝癌HepG2细胞增殖、凋亡以及侵袭、迁移能力的影响

Effect of miR-98 on proliferation,apoptosis,invasion and migration of hepatocellular carcinoma HepG2 cells

目的研究miR-98对肝癌Hep G2细胞增殖、凋亡和侵袭、迁移能力的影响及其可能机制。方法将miR-98mimics、mimics-NC、miR-98inhibitor、inhibitor-NC瞬时转入肝癌Hep G2细胞内,应用噻唑盐(MTT)法、流式细胞仪、Transwell小室实验检测miR-98对肝癌Hep G2细胞增殖、凋亡以及侵袭、迁移能力的影响,进一步用Western blot法检测各组Bcl-2蛋白的表达水平。结果 MTT实验表明miR-98过表达后,肝癌细胞的增殖能力明显低于对照组;Annexin VFITC/PI凋亡实验证实上调miR-98表达后,细胞的凋亡率较对照组升高;Transwell小室实验表明上调miR-98可使肝癌细胞的侵袭、迁移能力减弱。而当miR-98被抑制后,肝癌Hep G2细胞的增殖及侵袭、迁移能力则明显增强,凋亡率则下降。Western blot实验检测发现miR-98过表达后,Bcl-2的表达降低。结论 miR-98可能在肝癌的发生、发展中发挥着抑癌基因的作用;miR-98可能通过下调Bcl-2的表达,促进肝癌Hep G2细胞凋亡。

Objective To investigate the potential mechanism of miR-98’s effects on proliferation, apoptosis, and invasion / migration of HepG2 hepatocellular carcinoma （HCC） cells. Methods miR-98mimics, mimics-NC, miR-98 inhibitor and inhibitor-NC were transiently transfected into HepG2 cells. Effects of miR-98 on proliferation,ap-optosis, invasion / migration of HepG2 cells were determined by MTT assay, flow cytometer, and Transwell assay. Western blot was then performed to determine Bcl-2 expression levels in each group. Results MTT assay demon-strated that proliferation rate of HepG2 cells in miR-98 overexpressing group was significantly lower compared with control groups. Annexin V-FITC / PI revealed that apoptosis was more prominent in miR-98 overexpressing group compared with control groups. Transwell assay revealed that invasion and migration were significantly attenuated in miR-98 overexpressing group compared with control groups. Western blot demonstrated that Bcl-2 expression level was lower in miR-98 overexpression group compared with control groups. Conclusion miR-98 serves as a suppres-sor in the development of HCC; miR-98 promotes apoptosis of HCC cells by down regulating expression of Bcl-2.