自噬相关蛋白LC3-Ⅱ在上皮性卵巢癌中的表达及临床意义

Expression and clinical significance of autophagy-related LC3-Ⅱ in epithelial ovarian cancer

目的本研究检测微管轻链Ⅰ蛋白3-Ⅱ型(LC3-Ⅱ)在不同卵巢肿瘤组织中的表达情况,探讨其与浆液性卵巢癌及粘液性卵巢癌发生、发展的相关性和意义。方法选取204例上皮性卵巢肿瘤标本,其中,卵巢浆液性囊腺瘤46例,交界性浆液性肿瘤28例,浆液性囊腺癌40例,黏液性囊腺瘤32例,交界性黏液性囊腺瘤30例,黏液性囊腺癌28例,另选取正常卵巢上皮组织28例作为对照。使用SP法检测正常卵巢上皮组织、上皮性卵巢肿瘤组织中LC3-Ⅱ的表达,并结合浆液性癌、黏液性癌临床病理因素进行分析。结果1正常卵巢上皮中LC3-Ⅱ无表达,卵巢良性浆液性囊腺瘤、交界性浆液性肿瘤及浆液性癌中LC3-Ⅱ的阳性率分别为4.5%、71.0%、95.0%,交界性浆液性肿瘤和浆液性癌中LC3-Ⅱ的表达明显高于良性浆液性囊腺瘤,差异有统计学意义(P<0.01)。卵巢良性黏液性囊腺瘤、交界性黏液性肿瘤及黏液性癌中LC3-Ⅱ的阳性率分别为6.7%、66.7%、92.9%,交界性浆液性肿瘤和浆液性癌中LC3-Ⅱ的表达明显高于良性浆液性囊腺瘤,差异有统计学意义(P<0.01);2在40例卵巢浆液性癌、28例卵巢黏液性癌中,LC3-Ⅱ表达与患者年龄、FIGO分期及病理学分级等临床病理特征均无关。结论 LC3-Ⅱ在卵巢上皮性交界性肿瘤、癌中表达增高,自噬活性增强,可能与卵巢上皮性恶性肿瘤的进展有关。

Objective To investigate the expression of microtubule-associated protein light chain 3-Ⅱ（LC3-Ⅱ） in the epithelial ovarian carcinoma and explore their correlations to the tumorigenesis and development of epithelial o-varian carcinoma. Methods 204 specimens with different-type epithelial ovarian tumors,including 46 with serous cystadenoma,28 with borderline serous cystadenoma,40 with serous cystadenocarcinoma,32 with mucinous cystade-noma,32 with borderline mucinous cystadenoma,28 with mucinous cystadenocarcnoma,were selected in our study. Immunohistochemistry was employed to detect the expression of LC3-Ⅱ. The correlations between expression of LC3-Ⅱ and both the pathological features and clinical manifestations of 68 epithelial ovarian cancer patients were analyzed. Results The positive rates of LC3-Ⅱ were significantly higher in epithelial ovarian cancinoma and bor-derline ovarian tumors than benign epithelial ovarian tumors. （In ovarian serous tumor,95% and 71% vs 4. 5% ,P〈 0. 01;in ovarian mucinous tumor, 92. 9% and 66. 7% vs 6. 7% ,P 〈 0. 01） ② In the serous cystadenocarcinoma and mucinous cystadenocarcnoma,the expression of LC3-Ⅱ was not correlated to age, FIGO stage and histological grade. Conclusion Expression of LC3-Ⅱ is up-regulated in epithelial ovarian cancer tissues. The increase of au-tophagic capacity may relate to the development of epithelial ovarian cancer.