摘要
目的探讨不同病因所致肝衰竭患者外周血单核细胞HLA-DR基因mRNA表达及其意义。方法随机选取肝衰竭患者40例,其中嗜肝病毒感染10例、药物性肝损伤10例、酒精性肝损伤10例、自身免疫性肝病10例。10名健康对照为本院健康志愿者。应用RT-PCR法检测各组患者外周血单核细胞HLA-DR基因mRNA表达,并动态观察HLA-DR基因mRNA表达的变化,及其与病程发展及临床症状的关系。结果 RT-PCR结果显示嗜肝病毒感染组、正常对照组、药物损伤组、酒精损伤组、自身免疫组HLA-DR基因mRNA表达分别为(137.51±5.22)、(18.14±1.29)、(19.64±2.03)、(11.25±1.22)、(26.61±2.31),且呈逐渐降低趋势,组间差异均有统计学意义(P<0.05)。对不同病因组患者单核细胞HLA-DR mRNA表达进行动态观察发现,17例死亡患者HLA-DR mRNA表达持续下降直至患者死亡;另外23例患者,经治疗各组HLA-DR mRNA表达逐渐恢复至接近正常水平,临床症状同步改善。结论外周血单核细胞HLA-DR mRNA表达与肝功能衰竭的严重程度密切相关。
Objective To investigate the HLA-DR mRNA expression from peripheral blood mononuclear cells （PBMCs）of liver failure patients with different pathogenesis.Methods Forty patients with liver failure from 2010 to 2014 in our hospital were enrolled and divided into 4 groups according to pathogenesis. Meanwhile, 10 persons undergoing health examination were involved as control.Expression of HLA-DR mRNA from PBMCs was detected by real time-polymerase chain reaction （RT-PCR）at day 1 ,4,8,12,16,20 after hospitalization,to analyze its relevance with liver failure progress and clinical symptoms.Results There was significant difference in HLA-DR mRNA expressions among 4 groups.The level of HLA-DR mRNA decreased continuously without amelioration in 17 died patients,while that increased gradually to normal level with symptom improved in other 23 survived patients.Conclusion Expression of HLA-DR mRNA from PBMCs is closely related to severity of liver failure,which might be a valuable parameter to evaluate severity and prognosis of liver failure in clinic practice.
出处
《肝脏》
2015年第3期198-200,共3页
Chinese Hepatology
