组织工程三要素结合领域在软骨修复的研究进展

PROGRESS ON COMBINATION FIELDS OF THREE TISSUE ENGINEERING ELEMETS FOR CARTILAGE REPAIR

目的对软骨组织工程三要素——细胞、支架、生长信息三者的结合领域:细胞-支架修复技术、无细胞-基于支架的修复技术及无支架-基于细胞的修复技术进行综述。方法查阅近年国内外与软骨组织工程三要素结合领域相关的文献,并进行分析总结。结果细胞-支架修复技术如基质诱导自体软骨细胞移植,预先将软骨细胞固定,可提高其移植生存率;无细胞-基于支架的修复技术通过诱导剂促进自身细胞聚集修复,效果良好;无支架-基于细胞的修复技术最接近软骨胚胎发育过程,可避免支架降解产物毒性作用。结论细胞-支架、无细胞-基于支架、无支架-基于细胞的软骨组织工程技术提供了更接近自然软骨组织的修复方式,发展前景广阔。

Objective To summarize the tissue engineering techniques for cartilage repair on the combination fields of the three elements of tissue engineering：cells,scaffolds and signals.Methods The literature on cell-scaffoldbased cartilage repair techniques,cell-free scaffolds,and scaffold-free approaches was reviewed and summarized.Results The cell-scaffold-based cartilage repair techniques such as matrix-induced autologous chondrocyte implantation（chondrocytes are seeded on the scaffold） are able to enhance the survival of the cells;cell-free scaffolds can promote cell recruitment with chemoatractants;and scaffold-free approaches have better hyaline-like properties and can avoid the toxic effect of scaffold degradation products.Conclusion Combination fields of the three elements of tissue engineering provide a more biomimetic environment for cartilage repair and have broad prospects.