摘要
目的对比人抗狂犬病病毒IgG抗体定量测定酶联免疫试验(ELISA)试剂盒Autobio RABV-IgG ELISA、快速荧光灶抑制试验(RFFIT)以及BIO-BAD公司生产的PLATELIA^(TM)RABIESⅡKIT,检测狂犬病疫苗免疫后人血清抗狂犬病病毒IgG抗体水平的差异。方法收集狂犬病疫苗免疫前、免疫后临床血清样本共110份,使用RFFIT确定阳性标本(≥0.5 IU/ml)46份、阴性标本(<0.5 IU/ml)64份,分别用Autobio RABV-IgG ELISA及PLATELIATM RABIESⅡKIT同时对人抗狂犬病病毒IgG抗体进行测定,对比3种检测系统对人抗狂犬病病毒IgG抗体检测结果的阴性符合率、阳性符合率及—致性。结果 PLATELIA^(TM)RABIESⅡKIT与RFFIT的符合率为92.7%;Autobio RABV-IgG ELISA与RFFIT的符合率为91.8%,与PLATELIA^(TM)RABIESⅡKIT的符合率为97.3%。结论 3种检测系统之间呈现良好的符合率,Autobio RABV-IgG ELISA可用于人抗狂犬病病毒IgG抗体检测。
Objective To compare Autobio RABV -IgG Enzyme linked immunosorbent assay( ELISA), rapid fluorescent focus inhibition test (RFFIT) and PLATELIATM RABIES Ⅱ KIT produced by BIO - RAD Co. , Ltd. for determination of human anti -rabies virus IgG antibodies. Methods Total 110 serum samples from individuals vaccinated and non- vaccinated by ra- bies vaccines were collected. The rabies virus neutralizing anti - bodies (RVNAs) were determined by RFFIT. The RVNAs of 46 serum samples were positive ( ≥0.5 IU/ml). The RVNAs of 64 serum samples were negative( 〈0.5 IU/ml). The serum samples were tested the anti - rabies virus IgG antibodies by PLATELIATM RABIES Ⅱ KIT and Autobio RABV - IgG ELISA to investigate the negative and positive consistency of three detecting systems. Results The agreement of PLATELIATM RABIES Ⅱ KIT compared to RFFIT was 92.7%. The agreements of Autobio RABV - IgG ELISA compared to RFFIT and PLATELIATM RABIES II KIT were 91.8% and 97.3%. Conclusion Three systems detecting RVNAs showed satisfied consistency. Auto- bio RABV -IgG ELISA can be used to detect human anti -rabies virus IgG antibodies.
出处
《中国卫生检验杂志》
CAS
2015年第8期1172-1174,共3页
Chinese Journal of Health Laboratory Technology
基金
2014河南省科技攻关项目(142102310389)
关键词
狂犬病病毒
抗体
酶联免疫吸附试验
快速荧光灶抑制试验
Rabies virus
Antibody
Enzyme linked immunosorbent assay
Rapid fluorescent focus inhibition test