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七氟醚麻醉对大鼠海马载脂蛋白E mRNA表达的影响 被引量:5

Effect of sevoflurane anesthesia on expression of hippocampal apolipoprotein E mRNA of rats
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摘要 目的 探讨七氟醚麻醉对大鼠海马载脂蛋白E(ApoE) mRNA表达的影响.方法 健康雄性SD大鼠60只,体重300~ 400 g,15周龄,采用随机数字表法分为2组(n=30):50%氧气组(C组)、3.2%七氟醚麻醉组(S组).C组吸入50%氧气2h,S组吸入50%氧气+3.2%七氟醚2h.于麻醉前和麻醉后24和72 h时行Morris水迷宫实验;于麻醉后2、24和72 h时取海马组织,采用RT-PCR法测定ApoE mRNA的表达水平.结果 与C组比较,S组麻醉前后逃避潜伏期、原平台象限停留时间百分比、穿越次数百分比和运动距离百分比差异无统计学意义(P>0.05),麻醉后海马ApoE mRNA表达上调(P<0.05);2组逃避潜伏期、原平台象限停留时间百分比、穿越次数百分比和运动距离百分比麻醉前后比较差异均无统计学意义,海马ApoE mRNA表达水平麻醉后各时点比较差异无统计学意义(P>0.05).结论 七氟醚诱发的大鼠认知功能障碍与上调海马ApoE mRNA的表达无关. Objective To investigate the effect of sevoflurane anesthesia on the expression of hippocampal apolipoprotein E (ApoE) mRNA of rats.Methods Sixty healthy male Sprague-Dawley rats,weighing 300-400 g,aged 15 weeks,were randomly divided into 2 groups (n=30 each) using a random number table:50% oxygen group (group C) and 3.2% sevoflurane group (group S).The rats in group C inhaled 50% oxygen for 2 h,while those in group S inhaled 3.2% sevoflurane in 50% oxygen for 2 h.Morris water maze test was carried out before anesthesia and at 24 and 72 h after anesthesia.At 2,24 and 72 h after anesthesia,hippocampal specimens were obtained for determination of the expression of ApoE mRNA by RT-PCR.Results Compared with group C,no significant change was found in the escape latency,time of staying at the original platform quadrant,frequency of crossing the original platform,and swimming distance before and after anesthesia,and the expression of ApoE mRNA was up-regulated after anesthesia in group S.There was no significant difference in the escape latency,time of staying at the original platform quadrant,frequency of crossing the original platform,and swimming distance before and after anesthesia,and expression of ApoE mRNA at each time point after anesthesia between the two groups.Conclusion Cognitive dysfunction induced by sevoflurane has no relationship with the up-regulated expression of ApoE mRNA in the hippocampus of rats.
出处 《中华麻醉学杂志》 CAS CSCD 北大核心 2015年第2期172-174,共3页 Chinese Journal of Anesthesiology
基金 国家自然科学基金(81360180) 贵州省科技厅联合基金(黔科合J字LKZ[2011]29)
关键词 麻醉药 吸入 认知障碍 载脂蛋白E类 Anesthetics,inhalation Cognition disorders Apolipoproteins E
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参考文献9

  • 1Tang N, Ou C, Liu Y, et al. Effect of inhalational anaesthetic on postoperative cognitive dysfunction following radical rectal re- section in elderly patients with mild cognmve impairment[ J]. J Int Med Res,2014,42(6) : 1252-1261.
  • 2Xu JH, Zhang TZ, Peng XF, et al. Effects of sevoflurane before cardiopulmonary bypass on cerebral oxygen balance and early postoperative cognitive dysfunction[J]. Neurol Sci, 2013, 34(12) :2123-2129.
  • 3Kim J, Yoon H, Basak J, et al. Apolipoprotein E in synaptic plasticity and Alzheimer' s disease: potential cellular and molec- ular mechanisms[J]. Mol Cells,2014,37( 11 ) :767-776.
  • 4Hu N, Guo D, Wang H, et al. Involvement of the blood-brain barrier opening in cognitive decline in aged rats following orthope- dic surgery and high concentration of sevoflurane inhalation[J]. Brain Res,2014,1551 : 13-24.
  • 5陈龙,左明章,刘功俭,陈西艳,张岩,成勤,张茂银.七氟醚麻醉对幼鼠海马c-Jun氨基末端激酶表达和神经细胞凋亡的影响[J].中华麻醉学杂志,2011,31(5):563-565. 被引量:6
  • 6郑淑文,朱昭琼,马欢欢,张超,周军.哺乳期大鼠反复七氟醚麻醉对远期认知功能的影响[J].中华麻醉学杂志,2013,33(6):669-671. 被引量:7
  • 7Gutierrez-Lerma AL, Ordaz B, Pena-Ortega F. Amyloid Beta peptides differentially affect hippocampal theta rhythms in vitro [ J ]. Int J Pept,2013,328140.
  • 8Zhao W, Dumanis SB, Tamboli TY, et al. Human APOE geno- type affects intraneuronal AI31-42 accumulation in a lentiviral gene transfer model[J]. Hum Mol Genet,2014,23(5) :1365-1375.
  • 9杜敏逸,刘毓和,吴新民.反复吸入七氟烷对老年大鼠认知功能的影响[J].中华麻醉学杂志,2009,29(3):233-235. 被引量:20

二级参考文献34

  • 1陈松芳,黄汉津,王小同.NMDA受体NR1亚单位与学习记忆[J].中国行为医学科学,2006,15(1):91-92. 被引量:16
  • 2刘毓和,吴新民,杜敏逸.咪唑安定、异丙酚和氯胺酮对老年大鼠认知功能的影响[J].中华麻醉学杂志,2006,26(4):315-317. 被引量:51
  • 3Parikh SS, Chung F. Postoperative delirium in the elderly. Anesth Analg, 1995, 80:1223-1232.
  • 4Ancelin ML, De Roquefeuil G, Ritehie K. Anesthesia and postoperative cognitive dysfunction in the elderly: a review of clinical and epidemiological observations. Rev Epidemiol Same Publique, 2000,48 : 459-472.
  • 5Patel SS, Goa kL. Sevoflurane. A review of its pharmacodynamic and pharmacokinetic properties and its clinical use in general anaesthesia. Drugs, 1996,51 : 658-700.
  • 6Crawford MW, Lerman J, Pilato M, et al. Haemodynamic and organ blood flow responses to sevoflurane during spontaneous ventilation in the rat : a dose-response study. Can J Anaesth, 1992,39 : 270-276.
  • 7Crawford MW, Lerman J, Saldivia V, et al. Hemodynamic and organ blood flow responses to halothane and sevoflurane anesthesia during spontaneous ventilation. Anesth Analg, 1992, 75:1000-1006.
  • 8Shimizu E, Tang YP, Rampon C, et al. NMDA receptor-dependent synaptic reinforcement as a crucial process for memory consolidation. Science, 2000, 290: 1170-1174.
  • 9Wilson MA, Tonegawa S. Synaptic plasticity, place cells and spatial memory: study with second generation knockouts. Trends Neurosci, 1997, 20:102-106.
  • 10Bliss TV. Young receptors make smart mice. Nature, 1999, 401: 25- 27.

共引文献29

同被引文献25

  • 1Xu T,Bo L,Wang J,et al.Risk factors for early postoperative cognitive dysfunction after non-coronary bypass surgery in Chinese population[J].J Cardiothorac Surg,2013(8):204.
  • 2Shen X,Liu Y,Xu S,et al.Early life exposure to sevoflurane impairs adulthood spatial memory in the rat[J].Neurotoxicology,2013(39):45-56.
  • 3Fujimoto S,Ishikawa M,Nagano M,et al.Influence of neonatal sevoflurane exposure on nerve development-related microRNAs and behavior of rats[J].Biomed Res,2015,36(6):347-355.
  • 4Hayashi H,Campenot RB,Vance DE,et al.Apolipoprotein Econtaining lipoproteins protect neurons from apoptosis via a signaling pathway involving low-density lipoprotein receptor-related protein-1[J].J Neurosci,2007,27(8):1933-1941.
  • 5Alzate O,Osorio C,De Kroon RM,et al.Differentially charged isoforms of apolipoprotein E from human blood are potential biomarkers of Alzheimer's disease[J].Alzheimers Res Ther,2014,6(4):43.
  • 6Chang Y,Christine F,Dominic H,et al.APOE interacts with age to modify rate of decline in cognitive and brain changes in Alzheimer's disease[J].Alzheimer's&Dementia,2014,10(3):336-348.
  • 7秦斌,曾湘豫,Siest G.Alzheimer病脑脊液载脂蛋白E的定量分析[J].中华神经科杂志,1999,32(5):268-269.
  • 8Takahisa K,Xu H,Bu G,et al.ApoE and AβAlzheimer's disease:accidental encounters or partners[J].Neuron,2014,81(4):740-754.
  • 9Xie Z,Xu Z.General anesthetics andβ-amyloid protein[J].Prog Neuropsychopharmacol Biol Psychiatry,2013(47):140-146.
  • 10刘芬,闫荣.Aβ_(1-40)对PC12细胞突触素表达的影响[J].中风与神经疾病杂志,2010,27(9):815-818. 被引量:6

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