七氟醚麻醉对大鼠海马载脂蛋白E mRNA表达的影响

Effect of sevoflurane anesthesia on expression of hippocampal apolipoprotein E mRNA of rats

目的 探讨七氟醚麻醉对大鼠海马载脂蛋白E（ApoE） mRNA表达的影响.方法 健康雄性SD大鼠60只,体重300～ 400 g,15周龄,采用随机数字表法分为2组（n=30）：50％氧气组（C组）、3.2％七氟醚麻醉组（S组）.C组吸入50％氧气2h,S组吸入50％氧气＋3.2％七氟醚2h.于麻醉前和麻醉后24和72 h时行Morris水迷宫实验;于麻醉后2、24和72 h时取海马组织,采用RT-PCR法测定ApoE mRNA的表达水平.结果 与C组比较,S组麻醉前后逃避潜伏期、原平台象限停留时间百分比、穿越次数百分比和运动距离百分比差异无统计学意义（P＞0.05）,麻醉后海马ApoE mRNA表达上调（P＜0.05）;2组逃避潜伏期、原平台象限停留时间百分比、穿越次数百分比和运动距离百分比麻醉前后比较差异均无统计学意义,海马ApoE mRNA表达水平麻醉后各时点比较差异无统计学意义（P＞0.05）.结论 七氟醚诱发的大鼠认知功能障碍与上调海马ApoE mRNA的表达无关.

Objective To investigate the effect of sevoflurane anesthesia on the expression of hippocampal apolipoprotein E （ApoE） mRNA of rats.Methods Sixty healthy male Sprague-Dawley rats,weighing 300-400 g,aged 15 weeks,were randomly divided into 2 groups （n=30 each） using a random number table：50% oxygen group （group C） and 3.2% sevoflurane group （group S）.The rats in group C inhaled 50% oxygen for 2 h,while those in group S inhaled 3.2% sevoflurane in 50% oxygen for 2 h.Morris water maze test was carried out before anesthesia and at 24 and 72 h after anesthesia.At 2,24 and 72 h after anesthesia,hippocampal specimens were obtained for determination of the expression of ApoE mRNA by RT-PCR.Results Compared with group C,no significant change was found in the escape latency,time of staying at the original platform quadrant,frequency of crossing the original platform,and swimming distance before and after anesthesia,and the expression of ApoE mRNA was up-regulated after anesthesia in group S.There was no significant difference in the escape latency,time of staying at the original platform quadrant,frequency of crossing the original platform,and swimming distance before and after anesthesia,and expression of ApoE mRNA at each time point after anesthesia between the two groups.Conclusion Cognitive dysfunction induced by sevoflurane has no relationship with the up-regulated expression of ApoE mRNA in the hippocampus of rats.