生长抑制因子4在子宫内膜癌中的表达及意义

Expression of inhibitor of growth family member 4 in endometrial cancer and its significance

目的:检测生长抑制因子4(ING4)基因在不同子宫内膜癌中的表达情况,并探讨其表达与子宫内膜癌发生、发展及预后的关系。方法:采用RT-PCR方法和免疫组化S-P法检测25份正常的增生期子宫内膜、15份不典型增生的子宫内膜及45份子宫内膜癌组织中ING4的表达情况,观察结果并进行统计学处理。结果:免疫组化结果显示,ING4在增生子宫内膜中表达最多,在不典型增生的子宫内膜组织中的表达显著低于增生子宫内膜,在子宫内膜癌组织中的表达显著低于不典型增生组织,3组比较,差异有统计学意义(P<0.05)。RT-PCR结果与免疫组化测定结果一致,ING4 mRNA的表达在增生期子宫内膜组织、不典型增生子宫内膜组织、子宫内膜癌组织中逐渐降低,3组比较,差异有统计学意义(P<0.05)。在子宫内膜癌组织中,ING4的表达与组织学分级显著相关(P<0.05),与肌层浸润深度、淋巴结转移无关(P>0.05),但其表达与组织病理学分期的关系尚需进一步研究。结论:ING4在子宫内膜癌和不典型增生组织中的表达有不同程度的丢失,ING4的低表达促进了肿瘤的发生。检测ING4可能成为子宫内膜癌早期诊断及预测癌前病变的重要指标之一。

Objective： To detect the expressions of inhibitor of growth family member 4 （ING4） in different endometfial lesions, explore the relationship between ING4 and oneogenesis, development and prognosis of endometrial cancer. Methods： RT-PCR and immu- nohistochemical SP method were used to detect the expressions of ING4 in 25 cases with normal endometrium during the proliferative phase, 15 cases with endometrium of atypical hyperplasia, and 45 cases with endometrial cancer, the results were observed and analyzed statistical- The results of immunohistochemical SP method showed that the expression level of INGA in normal endometrium during the pro-liferative phase was the highest, the expression level of ING4 in endometrium of atypical hyperplasia was significantly lower than that in normal endometrium during the proliferative phase, and the expression level of ING4 in endometrial cancer was significantly lower than that in endometrium of atypical hyperplasia, there was statistieally signifieant difference among the three groups （P〈0. 05 ） . The results of RT- PCR and immunohistochemical SP method were the same, the expression level of ING4 mRNA in normal endometrium during the proliferative phase, endometrium of atypical hyperplasia, and endometrial cancer decreased gradually, there was statistically significant difference among the three groups （P〈0. 05 ） . In endometrial cancer, the expression level of ING4 was significantly correlated with histological grading （P〈 0. 05 ）, and it was not correlated with the depth of myometrial invasion and lymph node metastasis （P〉0.05）, the relationship between the expression level of ING4 and histopathological staging needed further studies. Conclusion： The expression levels of ING4 in endometrial cancer and endometrium of atypical hyperplasia are low to a certain degree, which promote the oncogenesis of endometrial cancer, ING4 may become to be an important index for early diagnosis of endometrial cancer and prediction of cervical precancerous lesion.