白菜型油菜春化基因BrFLC序列变异分析

Sequence variation of vernalization gene Br FLC in turnip rapes( Brassica rapa L. )

为寻找与冬春性相关的变异,以开发冬春性品种分子辅助标记,采用巢式和半巢式扩增法,对感温性和抗寒性强弱不同的5个白菜型油菜品种的Br FLC基因的第4外显子至第7外显子进行了特异性扩增。除Br FLC5无扩增结果外,其余3个基因均得到扩增。序列比对结果发现,3个Br FLC在第4外显子至第7外显子均存在有不同程度的等位变异,发生的主要等位变异有SNP和Indel两种类型。Br FLC1变异与冬春性相关性不大,与冬春性关系密切的变异主要发生在Br FLC2和Br FLC3上。Br FLC2中检测到1个Indel类型和5个SNP类型变异位点与冬春性有关。Br FLC3中检测到1个Indel类型和16个SNP类型变异位点与冬春性有关。

To better understand the variations related to winter and spring of Brassica rapa, 4 BrFLCs genes from 5 turnip rape cultivars （different in temperature sensitivity and cold hardiness） were investigated by nested and semi nested PCR. Primers were designed between the sequence of exon 4 to exon 7 in BrFLCs. Results showed that BrFLCl, BrFLC2 and BrFLC3 were amplified except BrFLCS. Sequence alignment analysis found some variations in the regions from exon 4 to 7 of the 3 BrFLCs. The main allelic variations were SNPs and Indels. Combining with their temperature sensitivity, variations in BrFLCl had little correlation to winterness, and those from BrFLC2 and BrFLC3 might have higher correlations. 1 Indel and 5 SNPs in BrFLC2, and 1 Indel and 16 SNPs in BrFLC3 might be associated with winterness.