微小RNA-194对基底样乳腺癌细胞HCC1937增殖、迁移侵袭及上皮-间充质转化的影响

Effects of microRNA-194 on proliferation, migration, invasion and epithelial-mesenchymal transition of basal-like breast cancer cell line HCC1937

目的 观察微小RNA-194（miR-194）对人基底样乳腺癌细胞（HCC1937）增殖、迁移侵袭能力及上皮-间充质转化（E MT）的影响.方法 利用慢病毒作载体（MOI=50）携带miR-194感染基底样乳腺癌细胞HCC1937为实验组,携带CON157慢病毒感染乳腺癌细胞HCC1937为阴性对照组,未做处理的HCC1937细胞为空白对照组.细胞计数试剂盒（CCK-8）法与Transwell小室实验分别检测HCC1937细胞增殖、迁移侵袭能力;Western blot方法检测EMT相关蛋白E-钙黏蛋白（E-cadherin）、N-钙黏蛋白（N-cadherin）的表达.结果 实验组细胞增殖能力为（44.81±0.11）％,显著低于阴性对照组为（104.29 ±0.13）％,空白对照组为（100±0）％（P ＜0.01）;实验组细胞迁移及侵袭力显著低于阴性对照组及空白对照组（P＜0.05）;E-cadherin蛋白：实验组（0.175 ±0.053）显著低于阴性对照组（0.516 ±0.032）、空白对照组（0.433±0.047,P＜0.05）;N-cadherin蛋白表达实验组（0.342±0.012）显著低于阴性对照组（0.137 ±0.007）、空白对照组（0.142 ±0.021,P＜0.01）.结论 miR-194能抑制基底细胞样乳腺癌细胞HCC1937增殖、迁移侵袭能力及EMT.

Objective To explore the role of microRNA-194 （miR-194） in proliferation,migration,invasion and the epithelial-mesenchymal transition （EMT） of human basal-like breast cancer cell line HCC1937.Methods Three groups were set up：experimental group [HCC1937 with lentivirus-miR-194-enhanced green fluorescent protein （EGFP）,MOI =50],negative control group （HCC1937with lentivirus-CON157-EGFP）,and blank control group （HCC1937 without lentivirus）.The miR-194 expression was detected by real-time quantitative reverse transcriptase-polymerase chain reaction （RT-qPCR）.Cell growth ability of HCC1937 was determined by cell counting kit-8 （CCK-8） assay.Cell migration and invasion ability of HCC1937 were mearsured by a Transwell assay,and the protein of epithelial-mesenchymal transition phenotype was measured by Western blotting.Results The expression of miR-194 in experimental group （432.93 ± 0.01） was higher than in negative control group （0.90 ±0.02） and blank control group （1） （P 〈0.01） The cells in experimental group grew more slowly [（44.81 ± 0.11）%] than negative control group [（104.29 ± 0.13）%] and blank control group [（100 ±0） %,P 〈0.01].The number of migration and invasion was both less in experimental group than in negative control group and blank control group （P 〈 0.05）.Western blotting revealed that the expression of E-cadherin in experimental group （0.175 ± 0.053） was significantly decreased as compared with negative control group （0.516 ± 0.032） and blank control group （0.433 ± 0.047,P 〈 0.05）,and the expression of N-cadherin in experimental group （0.342 ± 0.012） was significantly increased as compared with negative control group （0.137 ±0.007） and blank control group （0.142 ±0.021） （P 〈0.05）.Conclusion miR-194 can inhibit proliferation,migration and invasion of basal-like breast cancer cells （HCC1937）,and also affect EMT process in HCC1937 cells.