基质金属蛋白-9及组织蛋白酶S在人股动脉粥样硬化斑块中的表达

Expression of matrix metalloproteinase-9 and cathepsin S in atherosclerotic plaques of human femoral arteries

目的 探讨基质金属蛋白酶-9（MMP-9）及组织蛋白酶S（CatS）在动脉粥样硬化斑块中的表达.方法 收集人股动脉的粥样硬化斑块组织45例,正常脾动脉组织24例,分为稳定斑块组（20例）、不稳定斑块组（25例）以及对照组（24例）,采用免疫组织化学及反转录-聚合酶链反应（RT-PCR）检测各组标本中MMP-9和CatS及其mRNA的表达,以及两者间的关系.结果 MMP-9、CatS蛋白在平滑肌细胞、泡沫细胞、单核-巨噬细胞内有较多的表达,两者及其mRNA在各组中的表达差异有统计学意义（FMMP-9=168.448,P＜0.01;FMMP-9 mRNA=221.300,P＜0.01;FCats =273.246,P＜0.01;FCats mRNA=51.681,P＜ 0.01）.MMP-9、CatS蛋白及其mRNA在不稳定组（MMP-9蛋白：2.170±0.048,MMP-9 mRNA：1.588±0.085,CatS蛋白：2.231 ±0.050,CatS mRNA：1.411±1.104,P＜0.05）、稳定组（MMP-9蛋白：2.080±0.051,MMP-9 mRNA：1.346±0.071,CatS蛋白：2.097±0.045,CatS mRNA：1.280±0.058,P＜0.05）与对照组（MMP-9蛋白：1.959±0.011,MMP-9 mRNA：1.157 ±0.057,CatS蛋白：1.969 ±0.013,CatS mRNA：1.167±0.080,P＜0.05）中的表达依次降低,两者及其mRNA在稳定组、不稳定组中的表达均呈正相关（r稳定蛋白=0.690,P＜0.05;r稳定mRNA=0.739,P＜0.01;r不稳定蛋白=0.940,P＜0.01;r不稳定mRNA=0.820,P＜0.01）.结论 MMP-9、CatS蛋白的表达上调可能与动脉粥样硬化的发生及斑块的不稳定性有关.

0bjective To explore the expression of matrix metalloproteinase-9 （MMP-9） and cathepsin S （CatS） in antherosclerotic plaques.Methods Forty-five cases of human femoral arterial atherosclerotic plaques and 24 cases of nomal human splenic arterial samples （control group） were collected.The atherosclerotic plaques were divided into stable group （n =20） and unstable group （n =25）.The expression levels of MMP-9 and CatS were detected by immunohistochemical staining and reverse transcriptase-polymerase chain reaction （RT-PCR）.The correlations between MMP-9 and CatS were analyzed in stable and unstable groups.Results MMP-9 and CatS proteins were largely expressed in smooth muscle cells,foam cells and monocyte-macrophages.There was significant difference in the expression of MMP-9 and CatS protein and mRNA among groups （FMMP-9 =168.448,P〈 0.01;FMMP-9mRNA =221.300,P 〈0.01;FCatS =273.246,P 〈0.01;FCatS mRNA =51.681;P 〈0.01）.The expression of MMP-9 and CatS protein and mRNA was reduced in unstable group （MMP-9 protein：2.170 ± 0.048,MMP-9mRNA：1.588 ±0.085,CatS protein：2.231 ±0.050,CatS mRNA：1.411 ± 1.104,P 〈0.05）,followed by stable group （MMP-9 protein：2.080 ± 0.051,MMP-9 mRNA：1.346 ± 0.071,CatS protein：2.097 ±0.045,CatS mRNA：1.280 ±0.058,P 〈0.05）,and control group （MMP-9 protein：1.959 ±0.011,MMP-9 mRNA：1.157 ±0.057,CatS protein：1.969 ±0.013,CatS mRNA：1.167 ±0.080,P 〈 0.05）.There was a positive correlation between the expression of MMP-9 and CatS protein and mRNA in stable group and unstable group （rstable protein =0.690,P 〈 0.05;rstable mRNA =0.739,P 〈 0.01;runstableprotein=0.940,P〈0.01;rustablemRNA =0.820,P 〈0.01）.Conclusion The enhanced expression of MMP-9 and CatS protein was possible associated with the formation of atherosclerosis and the instability of plaques.