角度扫描型表面等离子体共振传感器抗噪声干扰的方法研究

Research on Anti-Noise-Interference Method of Angle Scanning Surface Plasmon Resonance Sensor

表面等离子体共振(SPR)传感器已广泛应用于生物学、医学、化学、药物筛选以及环境监测、食品安全等领域。但SPR传感系统检测样品时易受到来自光源、光电检测器等器件自身噪声及环境光噪声的影响,其中环境光噪声的影响尤为明显,因此SPR传感器通常需要在遮光环境下进行操作,不利于现场的快速检测。为此,在自行研制的角度扫描型SPR传感器基础上,重点研究环境光噪声的特性,提出一种统计平均滤波和低通滤波结合的数据处理方法来抑制日光灯照环境下的噪声干扰。检测磷酸盐(PBS)缓冲液的实验结果表明,该方法可使SPR传感器在日光灯照环境下准确检测,相对遮光测量结果的误差为0.0027%。该方法可有效提高角度扫描型SPR传感器抗环境光干扰能力,具有良好的应用和推广价值。

The surface plasmon resonance（SPR） sensor is widely used in biology, medicine, chemistry, drug screening, environmental monitoring, and food safety. However, the SPR sensor system can be easily affected by device self noise of optical source and photoelectricity detector etc, and ambient lights noise during detections,where the ambient light noise is particularly obvious. So the operation of SPR sensor needs to be in a shading environment, which is not conducive for rapid detections. To solve this problem, the characteristics of ambient light noise are analyzed on the basis of self-developed angle scanning surface plasmon resonance sensor. A data processing method, which combines an arithmetic average filtering and a low-pass filtering, is innovatively put forward to suppress the noise under fluorescent lights illumination. The experimental results of phosphate buffered saline（PBS） buffer detection shows that the proposed method can offer accurate detection under fluorescent lights illumination. The error of measurement results is 0.0027%, relative to the results in a shading measurement. The proposed method can effectively enhance the anti interference ability to ambient light of the angle scanning SPR sensor. Moreover, it has good application and popularization value.