脂氧素受体激动剂BML-111对野百合碱诱导肺动脉高压大鼠肺动脉压力及机制的研究

The effect of lipoxin receptor agonist BML-111 on rats pulmonary arterial hypertension and mechanisms induced by monocrotaline

目的:观察脂氧素受体激动剂BML-111对野百合碱(MCT)诱导肺动脉高压大鼠肺动脉压力的作用。方法:将SD大鼠24只随机分为C组、PH组、B组三组,PH组和B组大鼠行皮下注射野百合碱50μg/g,C组注射等量生理盐水,自野百合碱注射的当天起,B组每天腹腔注射1mg/kg·d BML-111连续4周。观察指标:平均肺动脉压力(MPAP)、右心室收缩压(RVSP)、右心室(RV)/[左室(LV)+室间隔(S)]、血浆内皮素ET及肿瘤坏死因子α(TNFα)。结果:PH组、B组与C组相比MPAP、RVSP、RV/(LV+S)、血浆ET及TNFα水平高于C组(P<0.05);B组与PH组相比,MPAP、RVSP、RV/(LV+S)、血浆ET及TNFα低于PH组(P<0.05)。结论:脂氧素受体激动剂BML-111可减轻野百合碱诱导肺动脉高压大鼠肺动脉压力,其机制可能与减轻血浆ET及TNFα水平,抑制肺动脉重构有关。

Objective ：To observe the the effect of BML‐111 on pulmonary arterial hypertension induced by monocrotaline and potential mechanisms .Methods ：24SD rats randomly divided into three groups ：C group ,PH group and B group ,Both PH group and B group processed subcutaneous injection of 50ug /g monocrotaline while C group received same volume of saline ,B group performed intraperitoneal injection of BML‐111 1mg · kg‐1 · d‐1 from the day when monocrotaline was injected .The observation ：MPAP ,RVSP ,RV /（LV ＋ S） ,the concentration of en‐dotheline（ET ） and tumor necrosis factor α（TNFα） in the plasma .Results ：the PH group and B group had higher level of MPAP ,RVSP ,RV /（LV ＋ S） ,the plasma concentration of ET and TNFαcompared with C group（P 〈 0 . 05） ,B group caused lower increase of MPAP , RVSP , RV/（LV ＋ S ） , the plasma concentration of ET and TNFαcompared with PH group（P 〈 0 .05） .Conclusion ：BML‐111 can ameliorate pulmonary arterial hypertension , part of the reason may be attribute to the reduction the inflammation and decreasing the pulmonary arterial remode‐ling .