抗小儿腹泻中药复方宝泻宁提取工艺的优化研究

Optimization of Extraction Process of Chinese Herbal Compound Bao-Xie-Ning for Anti-Diarrhea in Infant

为优化并建立抗小儿腹泻中药复方宝泻宁(BXN)的提取工艺,采用回流提取法,以吴茱萸碱、吴茱萸次碱、丁香酚、桂皮醛的转移率为考察指标,选择不同浓度乙醇为提取溶媒,选定溶媒含醇量、料液比、提取时间、提取次数为考察因素,采用L9(34)正交表进行BXN的提取工艺的优化。综合考虑各指标成分转移率及工业应用的方便、经济,经工艺验证,选定采用10倍量的φ=95%乙醇回流提取2次,每次提取2 h为优选工艺,该方法可有效提取BXN中的有效成分,4个指标成分的转移率均在80%以上,并具有稳定、经济、便捷的优点。

The study is to optimize and establish the extraction process of the Chinese herbal Compound Bao-Xie-Ning used for Anti-diarrhea in Infant.The process was optimized by taking the extraction per-centages of evodiamine,rutaecarpine,eugenol,cinnamic aldehyde as the evaluation parameters,consid-ering the concentration of ethanol （extraction solvent）,liquid-solid ratio,extraction time,extraction times as the affecting factor,and the extraction process of BXN optimized by L9 （3^4）orthogonal design. The results show that optimum condition is achieved when the concentration of ethanol is 95%,liquid-solid ratio is 10 （V/m）,the mixed herbs are extracted twice while 2 hours for each time.The established optimum extraction process is efficient for the extraction of BXN,and is a stable,economical and conven-ient method.