响应面法优化高吸油性淀粉制备工艺

Preparation optimization on high oil absorption microporous starch by response surface methodology

以马铃薯淀粉为原料,吸油率为评价指标,研究了双酶法分步处理淀粉,提高吸油率的最佳工艺方案.结果表明:α-淀粉酶处理后淀粉的吸油率可以达到86.17%,糖化酶的作用可以进一步提高吸油率.酶解的pH、温度、时间和酶使用量均能影响糖化酶的处理效果,响应面试验结果表明:当糖化酶添加量为1%、pH5.5、温度50℃、酶解时间12h时,测得微孔淀粉的吸油率为107.51%,与预测值108.16%的相对误差为0.60%,差异不显著,说明该模型拟合度好,优化后得到的微孔淀粉制备工艺准确可靠,较α-淀粉酶处理后淀粉的吸油率提高了21.34%.

With potato starch and oil absorption rate as raw material and evaluation index,the optimum techniques to improve oil absorption rate were determined with dual enzymatic stepwise handled starch.The result showed that the oil adsorption reached 86.17% when withα-amylase treatment.Moreover,glucoamylase treatment enhanced oil absorption.The pH,temperature,time of mixing and the enzyme adding quantity affected oil adsorption capacity.The results of the response surface showed that oil adsorption capacity of microporous starch reached 107.51%and the relative error was 0.60%compared with the predicted values 108.16%,when glucoamylase adding quantitys at 1%,pH 5.5,temperature at 50 ℃,enzymatic hydrolysis for 12 h.The difference was not significant compared with predict value,indicated that the model fitting degree was good.It is suggested that optimized microporous starch peeparation technology is accurate and reliable,improved 21.34% of oil absorption rate when compared to the starch treated byα-amylase.