桑黄多糖对类风湿性关节炎模型大鼠关节滑膜经典Wnt信号的影响

Effect of Phellinus Polysaccharide on Canonical Wnt Signaling in Synovium in Rats with Rheumatoid Arthritis

目的研究中药桑黄中活性成分多糖(PPS)对类风湿性关节炎(RA)模型大鼠的治疗作用和对RA模型大鼠滑膜组织经典Wnt信号的影响。方法将SD雄性大鼠随机分为3组:正常组、模型组和PPS组,每组10只。模型组和PPS组于每只大鼠右后足趾皮内注射0.1mL完全弗氏佐剂致炎制作RA模型,正常组于相同部位注射等量PBS;PPS组在造模后第8d给予50mg/kg PPS灌胃治疗,每日1次,至第32d,其余两组代之以等量生理盐水灌胃。造模后第16、20、24、28、32d对各组大鼠进行关节炎评分和足爪肿胀评分,造模后第28d采用real time qPCR检测各组大鼠滑膜中fibronectin表达,Wnt信号通路上游负调控基因SFRP1、2和通路关键基因β-catenin、C-myc、ccnd1的表达。结果 PPS灌胃治疗后,RA模型大鼠关节炎评分、足爪肿胀评分明显降低;与正常组相比,模型组大鼠滑膜中SFRP1、2表达明显降低,β-catenin、C-myc、ccnd1和fibronectin表达明显升高;但经PPS灌胃治疗后,PPS组滑膜中SFRP1、2表达明显升高,β-catenin、C-myc、ccnd1和fibronectin表达明显降低。结论PPS对RA模型大鼠滑膜中Wnt信号具有抑制作用,对RA模型大鼠具有一定的治疗作用。

Objective To investigate the role of Phellinus Polysaccharide（PPS）in activating canonical Wnt signaling in rats with rheumatoid arthritis（RA）.Methods Male SD rats were randomly divided into three groups（normal group,RA model group and PPS treated RA group）,each with 10 rats.The RA model rats were prepared through intradermal injection of 0.1mL complete Freund＇s adjuvant into the right rear toes of the rats.The PPS treated RA rats were given 50mg/kg PPS by gavage eight days after the introduction of RA.All rats were evaluated with arthritis score and paw swelling score at day 16,20,24,28,32.At day 28,the expressions of fibronectin gene,Wnt signal pathway negative regulation gene SFRP1,2and Wnt key geneβ-catenin,C-myc,and ccnd1 were detected by real time qPCR.Results PPS significantly reduced the arthritis score and paw swelling score of RA model rats.Lower levels of expression of SFRP1,2and higher levels of expression ofβ-catenin,C-myc,ccnd1 and fibronectin were found in the RA model rats compared with the normal controls.PPS increased the expression of SFRP1,2and decreased the expression ofβ-catenin,C-myc,ccnd1 and fibronectin in the RA rats.Conclusion PPS has significant therapeutic effect on RA model rats through inhibiting canonical Wnt signaling.