长链非编码RNA在压力超负荷引起的大鼠心肌肥厚中的差异表达

Expression profiles of long noncoding RNAs in rat myocardial hypertrophy induced by pressure overload

目的探讨长链非编码RNA(lncRNA)在腹主动脉缩窄术大鼠心肌肥厚和正常大鼠心肌中的差异表达。方法构建腹主动脉缩窄大鼠模型,术后4周测量大鼠超声心动图参数、左室质量指数,通过HE染色观察心肌细胞的面积,采用qRT-PCR法检测心肌肥厚相关因子ANF、β-MHC mRNA的表达水平,验证压力超负荷模型构建情况。采用lncRNA芯片技术检测lncRNA表达谱,筛选出具有差异性表达的lncRNA,并通过qRT-PCR验证芯片结果准确性。利用lncRNA与mRNA特异性表达的标准化信号强度,构建lncRNA与靶基因的共表达网络。结果大鼠心肌肥厚中共检测出6 969条lncRNA,其中显著上调表达80条,显著下调表达172条。经qRT-PCR检测显示,在大鼠心肌肥厚中MRAK134201下调表达,X89963上调表达,与芯片结果一致。构建共表达网络发现,与XR_008680共表达的蛋白编码基因111个。结论 lncRNA在压力超负荷性大鼠心脏组织中的表达谱发生变化,提示lncRNA可能在心肌肥厚的发生发展中具有一定的作用。

Objective To analyze the expressions of long noucoding RNA （lncRNA） in rat myocardial hypertrophy in- duced by abdominal aortic banding and in healthy rat hearts. Methods Rat models with myocardial hypertrophy in- duced by abdominal aortic banding were established. After 4 weeks, the echocardiographic data, left ventricular mass index and the myocyte cross-section were tested. Expressions of ANF and [3-MHC mRNA were determined with quanti- tative real-time polymerase chain reaction （qRT-PCR）. Differences of lncRNA expression profiles were inspected with IncRNA microarray and validated with qRT-PCR. Gene co-expression network was built with normalized signal intensi- ty of specifically expressed lncRNAs and mRNAs. Results We identified 6 969 lncRNAs, among which 80 were sig- nificandy up-regulated and 172 down-regulated. The expressions of lncRNA MRAK134201 and X89963 verified by qRT-PCR and microarray data were consistent. The network constructed from lncRNA XR_008680 was co-expressed with 111 coding mRNAs. Contusion Long noncoding RNAs are differentially expressed in rat cardiac hypertrophy models, indicating that lncRNAs might play a role in the pathogenesis of myocardial hypertrophy.