摘要
为了探索从棉油皂脚中提取植物甾醇的工艺条件。以皂脚为原料,通过单因素和正交实验优化最佳工艺条件,并对粗品进行纯化,以高效液相色谱法测定的甾醇含量为考察指标。植物甾醇提取的最佳工艺为:皂化料液比1∶3,90℃下皂化3 h,用乙酸乙酯进行萃取,料液比为1∶20,粗甾醇的提取率为75.6%,其中提取得到的β-谷甾醇和菜油甾醇的纯度分别为(66.5±0.47)%、(5.67±0.08)%,其经无水乙醇重结晶两次后,纯度可达91.8%。结果表明最佳提取工艺重现性较好;HPLC法测定甾醇含量结果准确可靠。
The process of extracting phytosterols from Cottonseed Soapstock has been established. Methods:The conditions have been optimized through single factor and orthogonal experiments. The crude extract was purified and the content of phytosterols was determined by HPLC. Results:Optimum extraction conditions were as follows:the Liquid-material ratio is 1∶3;saponification time is 3 h at 90℃;the extraction was carried out with ethyl acetate and the liquid-material ratio is 1∶20;the yield of the crude phytosterols is 75.6%and the purity ofβ-sitosterol and campesterol is (66.5±0.47)%and (5.67±0.08)%, respectively. The purity of total phytosterols has been enhanced from 70 % to 91.8 % after recrystallization with anhydrous ethanol twice. Conclusion:The optimized extraction process indicates satisfied repeatability and the method for determining phytosterols by HPLC is accurate and reliable.
出处
《食品研究与开发》
CAS
北大核心
2015年第6期45-48,共4页
Food Research and Development
基金
新疆建设兵团博士基金项目(2009JC14)
石河子大学重大科技攻关计划项目(gxjs2010-zdgg04)
关键词
棉油皂脚
植物甾醇
HPLC
提取
cottonseed soapstock
phytosterols
HPLC
extraction