盐酸克伦特罗酶标抗原的制备及鉴定

Synthesis and Identification of Immunogen Clenbuterol

选取辣根过氧化物为抗原标记酶,采用重氮化法将盐酸克伦特罗CLEN分别与牛血清蛋白(BSA)、卵清蛋白(OVA)偶联,合成包被抗原和免疫原,并通过紫外光谱扫描(UV)、SDS聚丙烯酞胺凝胶电泳法验证偶联成功。这为下一步获得针对盐酸克伦特罗类特异性单克隆抗体制备及其相应食品安全检测试剂盒开发奠定了基础。

Clenbuterol （CLEN）which was conjugated by horseradish peroxidase （HRP） was bound to bovine serum albumin （BSA） and ovalbumin （OVA） respectively by using diazotization, which aimed to synthesize artificial immunogenic antigen CLEN-BSA and artificial detection antigen CLEN-OVA. The results of UV scan , SDS-PAGE indicated that the artificial BSA and artificial detection antigen CLEN-OVA were synthesized successfully. The results demonstrated that the CLEN-BSA conjugate has immunogenicity which is good for next monoclonal antibody preparation and the research of ELISA kit.