针刺得气对寒凝证类痛经模型大鼠效应指标的作用

Effect of needling sensation in acupuncture treatment on uterine in primary dysmenorrheal rats of cold stagnation syndrome

目的观察针刺三阴交穴"得气"与"未得气"状态对寒凝证类痛经模型大鼠子宫收缩、微循环及扭体反应的影响。方法将96只动情间期雌性SD大鼠随机分为盐水对照组(对照组)、寒凝证类痛经模型组(模型组)、得气组与未得气组,每组24只。采用全身冷冻加雌二醇注射方法制备类痛经大鼠模型。得气组与未得气组造模成功后对大鼠双侧三阴交穴进行针灸刺激,观察大鼠子宫张力不同时间段变化、微循环多时间窗变化及扭体反应。结果与对照组相比,模型组子宫活动度显著增加。得气组的子宫收缩波个数及子宫活动度比模型组显著减少。模型组与未得气组的子宫微血管、毛细血管管径比对照组明显收缩;与模型组相比,得气组在各时间段微血管均扩张,毛细血管管径在针刺后40、50、60 min明显扩张(P<0.05)。与模型组相比,得气组扭体潜伏期明显延长、扭体次数减少、扭体评分降低(P<0.01)。结论针刺三阴交穴得气能缓解痛经模型大鼠子宫收缩,改善子宫微循环障碍,并具有一定的后续效应。

Objective To investigate the effect of needling Sanyinjiao point （SP6） on the uterine contractions, uterine sensation （de qi ） when acupuncturing at microcirculation and the body torsion reaction in primary dysmenorrheal （PD）rat model with cold stagnation syndrome. Methods 96 anestrous female SD rats were divided into four groups （n = 24 each） of saline control group （control group）, PD model with syndrome of cold stagnation group （ model group） , acupuncture treatment with obtaining ＂de qi ＂ sensation group（ deqi group）and acupuncture treatment without obtaining ＂de qi ＂sensation group （no deqi group）. Rat model with PD was induced by whole body freezing combined with intraperitoneal injection of estradiol benzoate. After modeling, bilateral Sanyinjiao points （SP6） of rats were stimulated by acupuncture, and the indexes of uterine contraction, uterine microcirculation and the body torsion reaction were recorded and analyzed. Results Compared with control group, the uterine activity mcreaseU significantly in model group. The number of uterine contraction wave and uterine activity decreased markedly in deqi group compared with model group. The diameter of capillaries and microvessels of uterine decreased significantly in both model group and no deqi group compared with control group, while microvessel relaxation at each time point and capillaries relaxation at 40, 50, 60 rain after acupuncture in deqi group were observed. Meanwhile, compared with model group, writhing times and scores decreased markedly with writhing latency inereasing in deqi group. Conclusion The effect of relieving uterine contraction and improving uterine microcirculation in deqi group maybe relies on obtaining needling sensation, which is the key of efficacy of acupuncture treatment.