微流控芯片非接触电导法测定僵蚕中草酸铵的含量

Content Determination of Ammonium Oxalate in Bombyx Batryticatus by Microfluidic Chip with Contactless Conductivity Method

目的：建立测定僵蚕中草酸铵含量的方法。方法：采用微流控芯片非接触电导法,探讨缓冲液种类和浓度、添加剂种类和浓度、进样时间和分离电压等因素对分离检测的影响。结果：优选缓冲体系为3 mmol/L三羟甲基氨基甲烷＋3 mmol/L柠檬酸（p H=3.0）、不加添加剂、进样时间为10 s、分离电压为2.0 kV。草酸铵质量浓度在10~150μg/ml范围内与峰面积呈良好线性关系（r=0.999 8）;检测限为3.0μg/ml;精密度、稳定性、重复性试验的RSD均〈2.0%;平均加样回收率为99.1%,RSD=1.4%（n=9）。结论：该方法简便快速、重复性好,为僵蚕的质量控制提供了一种新方法。

OBJECTIVE：To establish the method for the content determination of ammonium oxalate in bombyx batryticatus.METHODS：Microfluidic chip with contactless conductivity were conducted to detect the effects of types and concentration of buffer solution and additives,volume time and separation voltage on separation detection.RESULTS：The butter system was optimized as3 mmol/L Tris＋3 mmol/L citric acid（p H=3.0）with no additives,volume time of 10 s and the separation voltage of 2.0 kV.There was a good linear relationship between the mass concentration of ammonium oxalate and peak area in the range of 10-150 μg/ml（r=0.999 8）with the detection limit of 3.0 μg/ml;the RSDs of precision,stability and reproducibility tests were less than 2.0% and the average recovery was 99.1%（RSD=1.4%,n=9）.CONCLUSIONS：The method is simple,rapid and well reproducible and provides a new method for the quality control of bombyx batryticatus.