紫外可见分光光度法测定无花果叶提取物中总黄酮的含量

Content Determination of Total Flavonoids in Extracts from Ficus Carica Leaves by UV-visible Spectrophotometry

目的：建立测定无花果叶提取物中总黄酮含量的方法。方法：采用紫外可见分光光度法,以芦丁为对照品,采用亚硝酸钠-硝酸铝-氢氧化钠显色法,在510 nm波长处对无花果叶提取物的总黄酮含量进行测定。结果：芦丁质量浓度在0.05-0.50 mg/ml范围内与吸光度呈良好的线性关系（r=0.999 9）;精密度、稳定性、重复性试验的RSD均〈2%;平均加样回收率为98.68%,RSD=1.50%（n=9）。无花果叶黄酮提取物的总黄酮含量均值为9.47%。结论：该方法操作简便、准确,稳定性和重复性良好,适用于无花果叶提取物中总黄酮的含量测定。

OBJECTIVE：To establish a method for the content determination of total flavonoids in extracts from ficus carica leaves.METHODS：The content of total flavonoids in extract from ficus carica was determined by UV-visible spectrophometry with Al（NO3）3-Na NO2-Na OH color-test at the wavelength of 510 nm with the reference of rutin.RESULTS：There was a good linear relationship between the quality concentration of rutin and the absorbance in the range of 0.05-0.50 mg/ml（r=0.999 9）.The RSDs of precision,stability and reputability test were less than 2% and the average recovery was 98.68%（RSD=1.50%,n=9）.The average content of total flavonoids in extract from ficus carica leaves was 9.47%.CONCLUSIONS：The method is simple,accurate,reproducible and stable,and can be used for the content determination of total flavonoids in extract from ficus carica leaves.