摘要
目的:建立测定溴甲东莨菪碱片含量的方法。方法:采用高效液相色谱法。色谱柱为Shim-packVP-ODS,流动相为甲醇-0.5%庚烷磺酸钠溶液(含0.5%冰醋酸)(35∶75,V/V),流速为1.0 ml/min,检测波长为230 nm,柱温为室温,进样量为20μl。结果:溴甲东莨菪碱质量浓度在0.100 2~1.502 7 mg/ml范围内与峰面积呈良好的线性关系(r=0.999 9);精密度、稳定性、重复性试验的RSD均〈1.0%;平均加样回收率为99.66%,RSD为0.43%(n=9)。结论:本方法准确度高、操作简便,可用于溴甲东莨菪碱片的含量测定。
ABSTARCT OBJECTIVE:To establish HPLC method for the content determination of Methscopolamine bromide tablets.METHODS:HPLC was conducted.The column was Shim-packVP-ODS with the mobile phase of methanol-0.5% heptane sulfonic acid sodium solution(containing 0.5% acetic acid)(35∶75,V/V)at the flow rate of 1.0 ml/min;the wavelength was 230 nm,the temperature was room temperature and the volume was 20 μl.RESULTS:There was a good linear relationship between the quality concentration of methscopolamine bromide and peak area in the range of 0.100 2-1.502 7 mg/ml(r=0.999 9).The RSDs of precision,stability and reproducibly tests was all less than 1.0% and the average recovery was 99.66%(RSD=0.43%,n=9).CONCLUSIONS:The method is accurate,easy and can be used for the content determination of Methscopolamine bromide tablets.
出处
《中国药房》
CAS
北大核心
2015年第15期2126-2127,共2页
China Pharmacy