孤独症儿童血清中tPA和PAI-1的水平

Serum tPA and PAI-1 levels in autism children

目的探讨组织型纤溶酶原激活剂(tissue-type plasminogen activator,tPA)和纤溶酶原激活物抑制因子-1(plasminogen activator inhibitor-1,PAI-1)血清浓度与儿童孤独症的关系。方法依据《精神疾病诊断与统计手册-第四版(DSM-Ⅳ)》选择已明确诊断的孤独症儿童40例,并按照儿童期孤独症评定量表(The Children Autism Rating Scale,CARS)标准对孤独症儿童进行病情分级;另选40例健康儿童作为对照。采用酶联免疫吸附试验检测所有受试儿童血清中tPA和PAI-1水平。用SPSS 20.0统计软件做两组样本t检验和方差分析进行统计分析。结果病例组儿童血清tPA水平明显低于对照组儿童,而病例组儿童血清PAI-1水平显著高于对照组儿童,差异均具有统计学意义(P均<0.05)。按病情分级后,轻度孤独症儿童血清tPA水平与对照儿童相比有降低趋势,但差异无统计学意义(P>0.05),重度孤独症儿童与对照组儿童相比明显降低(P<0.05),轻、重度孤独症儿童之间的tPA水平差异无统计学意义(P>0.05)。轻、重度孤独症儿童血清PAI-1水平与对照儿童相比均明显升高(P<0.05),轻、重度孤独症儿童之间无明显差异(P>0.05)。此外,无论是对照组还是病例组均未发现tPA和PAI-1水平存在性别差异(P>0.05)。结论孤独症儿童血清tPA水平明显降低,而PAI-1水平明显升高,推测两者在孤独症的发病过程中可能具有重要作用。

Objective To evaluate the serum tissue-type plasminogen activator （tPA） and plasminogen activator inhibi tor-1（PAI-1） levels of children with autism and evaluate the relationship between tPA and PAI-1 levels of autism. Methods According to Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition （DSM-IV） and The Children Au- tism Rating Scale （CARS）,40 children were diagnosed as autistic cases and graded for the illness severity. 40 healthy chil- dren were enrolled as control. Levels of cytokines in serum were quantified by an enzyme-linked immunosorbent. With a SPSS 20.0 statistical software,t test and ANOVA were used to analyse the statistical significant. Results Serum tPA level of the children with autism was lower than that of control （P〈0.05）. Serum PAI-I level of the children with autism were significantly higher than that of the control children （P〈0.05）. According to the graded for the illness classification, the serum tPA level in the mild autism children had a decreased trend when compared with the children in the control group （P〉0.05） ,the difference was not significant. The tPA level in the severe autism children was significantly lower than that in control children （P〈0.05）. There was no significant difference in mild and severe autism children （P〉0.05）. Both of the PAl-1 levels of the mild and the severe autism children were significantly higher than that in control （P〈0.05）. There also was not statistically differences between the mild and severe autism children for the PAl-1 levels （P〉0.05）. The tPA and PAI-1 had no significant difference between male and female in both control and autism group （P〉0.05）. Conclusion The serum tPA level in children with autism is significantly lower than that in control,but serum PAI-1 of the levels is sig nificantly higher than that in control,both may play an important role in the pathogenesis of autism.