宫颈癌灶精细组织结构定性研究

Qualitative study on the fine structure of the cervical cancer

目的探讨宫颈癌灶精细组织结构,了解宫颈癌灶的组织成分。方法选取2014年6-10月在南方医科大学南方医院行宫颈活检及广泛性子宫切除术后的新鲜宫颈癌灶标本33例,分别采用HE染色、特殊染色及多种免疫组织化学染色对宫颈癌灶进行定性研究。结果 (1)宫颈癌灶中除各种不同分化程度的癌细胞外,还含有丰富的纤维结缔组织,包括胶原纤维及弹力纤维,未见平滑肌纤维;含有大量的血管,包块微小动静脉及微血管;淋巴管含量较多,未见淋巴结;部分血管及淋巴管腔内见癌细胞浸润。(2)除上述基本结构外,发现部分宫颈癌灶中存在神经纤维。特异性标记神经纤维的S-100抗体染色阳性有14例,阳性比例为42.42%;特异性标记交感神经的TH抗体染色阳性数有7例,标记副交感神经的VIP抗体染色阳性数有5例,两者与S-100阳性数比例分别为50%、35.71%;特异性标记新生神经的GAP-43抗体染色阳性有11例,其与S-100阳性数比例为78.57%。结论宫颈癌灶中除癌细胞、纤维结缔组织、血管及淋巴管等基本组织结构外,还存在神经纤维,且大部分神经为新生神经,交感神经略高于副交感神经。

Objective To investigate the fine structure of cervical cancer, in order to elucidate tissue components.Methods Thirty-three fresh specimens of cervical cancer were collected from cervical biopsy and radical hysterectomy from June to October 2014 at Nanfang Hospital, and were used for qualitative research by HE staining, special staining and a variety of immunohistochemical staining. Results 1.In addition to lots of cancer cells with different differentiation, cervical cancer was rich in fibrous connective tissue, including collagen and elastic fibers, no muscle fiber; it also contained a lot of blood vessels, including microvascular vessels;it contained many lymphatic vessels, no lymph nodes;lymphatic and vascular invasion was found in a part of cervical cancer. 2. In addition to the basic structure, nerve fibers were found in some cervical cancer.（1） The positive cases of nerve-specific marker S-100 were 14 and the ratio was42.42%.（2） The positive cases of sympathetic nerve-specific marker TH were 7, with the ratio of S-100 positive cases in50%. The positive cases of parasympathetic nerve-specific marker VIP were 5, with the ratio of S-100 positive cases in35.71%.（3） The positive cases of newborn nerve-specific marker GAP-43 were 11, with the S-100 positive in 78.57%.Conclusion In addition to the basic structure of the cancer cells, fibrous connective tissue, blood vessels and lymphatic vessels, cervical cancer also contains nerve fibers, and most of the nerve fibers are newborn nerve,with the content of sympathetic nerve slightly higher than parasympathetic nerve.