甲氨蝶呤片对正常大鼠血液生化指标的影响

Influence of Methotrexate Tablets on Normal Rat Blood Biochemical Indexes

目的探讨甲氨蝶呤片对正常大鼠血液生化指标的影响。方法选择SD大鼠40只,随机分为溶剂对照组、甲氨蝶呤组(1.05 mg/kg),各20只,雌、雄各半。连续灌胃相应药物65 d,观察大鼠一般状况、体重、脏器系数、血液生化指标。结果与溶剂对照组比较,第5周开始,甲氨蝶呤组个别大鼠出现轻微腹泻;甲氨蝶呤组雄性大鼠心脏、肺脏、胸腺、肾脏系数升高(P<0.05);雌性大鼠肝脏、脾脏、肾脏系数升高而胸腺、脑系数降低(P<0.05);甲氨蝶呤组雄性大鼠血清总胆红素(TBIL)升高、碱性磷酸酶(ALP)降低,雌性大鼠血清三酰甘油(TG)升高,天门冬氨酸氨基转移酶(AST)、ALP、磷酸肌酸激酶(CK)降低(P<0.05)。结论初步认为,甲氨蝶呤片对大鼠胃肠道、肾脏、肝脏、心脏、肺脏、胸腺、脾脏、脑功能有一定影响。

Objective To investigate the influence of Methotrexate （ MTX ） Tablets on blood biochemical indexes in the normal rats. Methods 40 SD rats were selected and randomly divided into the solvent control group and the MTX group （ 1. 05 mg/kg ） , 20 cases in each group, half male and half female. The continuous gavage of corresponding drugs lasted for 65 d. The general condition, body weight, visceral organ mass and blood biochemical indexes were observed. Results Compared with the solvent control group, in-dividual rats in the MTX group appeared mild diarrhea from the beginning of 5 th week. The coefficients of cardiac, lung, thymus and kidney in male rats of the MTX group were elevated （ P ﹤ 0. 05 ）; the coefficients of liver, spleen and kidney in female rats were increased and the coefficients of thymus and brain were decreased （ P ﹤ 0. 05 ） . The serum level of TBIL in male rats of the MTX group was increased and the ALP level was decreased; the serum TG level in female rats was increased and the serum AST, ALP and CK were decreased, the differences were statistically significant （ P ﹤ 0. 05 ） . Conclusion It is initially thought that MTX Tablets has certain influence on gastrointestinal tract, kidney, liver, heart, lung, spleen, thymus, brain function in rats.