DKK-3基因甲基化在胃癌病情及预后评估中的价值

Value of DKK-3 gene methylation for evaluating disease condition and prognosis in gastric cancer

目的研究胃镜活检组织Dickkopf相关蛋白(DKK-3)基因甲基化在胃癌病情及预后评估中的价值。方法采用甲基化特异性聚合酶链反应检测胃癌患者肿瘤组织、癌旁正常组织及健康人胃镜活检组织的DKK-3基因甲基化,比较不同临床病理因素中DKK-3基因甲基化的差异,分析3年生存率与DKK-3基因甲基化的关系。结果观察组研究对象肿瘤组织DKK-3基因甲基化率显著高于癌旁正常组织和健康对照组研究对象正常组织,差异有统计学意义(P<0.05),DKK-3基因甲基化率在癌旁正常组织和健康对照组研究对象正常组织差异无统计学意义(P>0.05)。观察组胃癌患者肿瘤组织中DKK-3基因甲基化率在性别、年龄、病变部位、肉眼形态、幽门螺杆菌感染和病理类型中差异无统计学意义(P>0.05),在分化程度、肿瘤直径、淋巴结转移、远处转移和TNM分期中差异有统计学意义(P<0.05)。DKK-3基因甲基化胃癌患者3年生存率为23.8%,未甲基化胃癌患者3年生存率为52.6%,DKK-3基因甲基化胃癌患者3年生存率显著高于未甲基化患者,差异有统计学意义(P<0.05)。结论胃癌患者胃镜活检组织DKK-3基因甲基化率越高则病情越重、预后越差,可作为胃癌病情及预后评估的标志物。

Objective To study the value of Dickkopf‐related protein‐3（DKK‐3） gene methylation by gastros‐copy biopsy for evaluating the disease condition and prognosis in gastric cancer .Methods The methylation specific PCR was used to detect the DKK‐3 gene methylation by gastroscopy biopsy in gastric cancer tissue ,paracancerous normal tissue and healthy controls .The DKK‐3 methylation rates were compared among different clinicopathological factors .Results The DKK‐3 methylation rate in the tumor tissues of the observation group was significantly higher than that in the paracancerous normal tissues of the observation group and the healthy contol group ,the differences were statistically significant（P〈0 .05） .The DKK‐3 methylation rates had no statistical differrences in the gender , age ,lesion position ,gross morphology ,Hp infection and pathological type in the cancerous tissue of the observation group ,but the differences in the differentiation degree ,caner diameter ,lymph node metastasis ,distant metastasis and TNM staging were statisticlly significant（P〈0 .05） .The 3‐year survival rate was 23 .8% in the gastric cancer pa‐tients with DKK‐3 methylation and 52 .6% in the patients without DKK‐3 methylation ,which indicated that the 3‐year survival rate in the patients with DKK‐3 methylation was significantly lower than that without DKK‐3 methyla‐tion ,the difference was statistically significant（P〈 0 .05） .Conclusion The higher the DKK‐3 methylation rate by gastroscopy biopsy ,the poor the prognosis ,so DKK‐3 methylation may serve as the marker for evaluating the disease condition and prognosis .