摘要
目的构建菖蒲属药用植物的鉴别方法。方法采用DNA条形码序列技术,对5种菖蒲属药用植物的4条候选DNA条形码序列(ITS2、rbc L、mat K、psb A-trn H)进行PCR扩增和测序,比较各序列的扩增和测序效率,对比分析种内、种间的遗传变异、分析barcoding Gap并构建聚类分析树。结果 4条候选DNA条形码序列PCR扩增和测序效率均为100%,ITS2序列对菖蒲属药用植物的鉴定成功率最高。结论基于ITS2序列的DNA条形码技术可以鉴定菖蒲属药用植物。
Objective To develope a method for identification of medicinal plants of the Acorus L.. Methods DNA barcoding technology was adopted and 5 species of Acorus L. medicinal plants of were sequenced（barcoded）for 4 candidate DNA barcode sequence（ITS2, rbc L, mat K, psb A-trn H）. The PCR amplification and sequencing efficiency, intra-specific and inter-specific divergence and barcoding gap were analyzed and cluster analysis tree was constructed. Results The amplification and sequencing efficiency of the 4 candidate DNA barcode was 100%. ITS2 sequence had the highest identification success rate. Conclusion The DNA barcoding technology based on ITS2 sequences can be used to correctly identify medicinal plants of Acorus L..
出处
《中南药学》
CAS
2015年第4期388-392,共5页
Central South Pharmacy
基金
国家科技重大专项课题(编号:2014ZX09304307-002)
湖南省自然科学基金项目(编号:13JJ3132)
