早产儿喂养不耐受肠道菌群多样性研究

Investigation of the intestinal microbial community diversity in preterm infants with feeding intolerance

目的采用变性梯度凝胶电泳聚合酶链反应(PCR-DGGE)技术从微生物生态学的角度分析比较喂养不耐受(FI)与健康早产儿肠道细菌群落结构的多样性及相似性。方法以2013年11月至2014年9月在第四军医大学附属唐都医院儿科新生儿病房诊断为FI的早产儿为FI组。选择与FI组胎龄、日龄、出生体重相匹配的非FI早产儿作为对照组。采集出现FI时和同时间段对照组的粪便标本,进行16SrDNAV3区扩增和变性梯度凝胶电泳(DGGE),从而分析比较两组间肠道菌群多样性指数及相似性。结果细菌多样性检测显示FI组的肠道菌群多样性指数香农-维纳指数(H)、丰度(S)、均衡度指数(E)和辛普森多样性指数(D)均低于对照组(均P<0.05);相似性矩阵图及聚类分析结果显示组内菌群相似性较组间高(P<0.05);PCA结果同聚类分析一致。结论肠道微生物群落多样性的改变及群落结构紊乱可能是引起早产儿FI的重要因素。

Objective To observe and compare the diversity and similarity of intestinal bacterial community structure of preterm infants who had feeding intolerance （ FI ） and those who were healthy from the perspective of microbial ecology using polymerase chain reaction-denaturing gradient gel electrophoresis （ PCR-DGGE） technology.Methods The preterm infants diagnosed as FI during December 2013 to September 2014 in neonatal wards of pediatric department of Tangdu Hospital of the Fourth Military University were recruited in FI group. The infants without FI but matched in gestational age, days of age and birth weight during the same period were taken in control group.The stool samples were collected in FI group when FI occurred and in the control group at the same period for conducting 16SrDNA V3 region amplification and DGGE, and thus the diversity and similarity of intestinal bacterial community structure between two groups were analyzed and compared.Results The results of bacterial diversity showed that H, S, E and D of FI group were lower than those of the control group （all P 〈0.05）.Similarity matrix and cluster analysis showed that the intra-group similarity of the flora was higher than inter-group similarity （P〈0.05）.Principal components analysis （PCA） was consistent with the cluster analysis.Conclusion The change of intestinal microbial community diversity and community structure disorder may be the important factor causing FI in preterm infants.